低能量光照對人類皮膚纖維母細胞及U-937單核球細胞之影響

The Effects of Low-level Light Irradiation on Human Dermal Fibroblasts and Monocytic U-937 Cells

馮如愛(Ju-Ai Feng)

指導教授 ： 李文婷

中原大學/工學院/醫學工程研究所/碩士(2006年)

https://doi.org/10.6840/cycu200700346

摘要

自從光生物刺激理論被提出後，光照治療成為醫學上常使用的治療方式，特別是在傷口癒合的治療。近年來，發光二極體(Light emitting diode, LEDs)逐漸取代雷射成為新一代光照治療所使用的光源。本實驗利用先前所獲得的光照刺激最佳參數，持續探討以紅光LEDs刺激不同培養方式之人類皮膚纖維母細胞(human dermal fibroblast, HDF)及人類淋巴瘤細胞(U-937)的影響。光源波長為630 nm，功率15 mW/cm2，細胞所接受之能量為4 J/cm2。以細胞計數方式分析細胞數之變化，發現光照刺激可促進HDF之增生；亦可透過刺激U-937細胞，間接促進HDF之增生。以Sirius Red- Fast green染色法分析HDF膠原蛋白之生合成，發現光照刺激可促進HDF膠原蛋白之分泌；亦可透過刺激U-937細胞，間接促進HDF膠原蛋白之生合成。最後，使用DCFDA螢光染劑，測量HDF活性氧分子(Reactive oxygen species, ROS)產生的變化，實驗結果發現光照刺激並未顯著提升ROS之產生。

關鍵字

膠原蛋白 ； 活性氧分子 ； 人類皮膚纖維母細胞 ； 發光二極體

並列摘要

After the theory of photomodulation was proposed, light therapy has been widely used in clinical treatments, particularly in wound healing. Lately, light emitting diodes (LEDs) have gradually replaced lasers and became new generation of light sources in the light therapy. Here we describe the effects of red-light irradiation on different ways of culturing human dermal fibroblasts (HDFs) and human lymphoma cells (U-937) by using the optimal stimulating parameters obtained previously. The LEDs used had a wavelength of 630 nm. Its irradiance was 15 mW/cm2 and the radiant exposure was 4 J/cm2. The change in cell numbers was analyzed by direct cell counting. Significant enhancement of HDFs proliferation was found after light irradiation. The growth of HDFs was also promoted by incubating HDFs in the culture supernatant of illuminated U-937 cells. The secretion of total collagen by HDFs was estimated by using Sirius red- Fast green stain. The increase of the collagen secretion by HDFs was observed after irradiation. The secretion of collagen was also enhanced by incubating HDFs in the supernatant of light-stimulated U-937 cells. By using DCFDA analysis for the generation of reactive oxygen species (ROS) by HDFs, no significant difference was observed after illumination.

並列關鍵字

collagen ； reactive oxygen species ； human dermal fibroblast ； light emitting diode

參考文獻

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