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  • 學位論文

用於捕捉與回收白血球的熱敏性聚丙烯纖維薄膜的研究

Study on thermo-sensitive polypropylene fibrous membranes for capturing and recovering white blood cells

指導教授 : 張雍
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摘要


本論文的研究目的為設計可用於捕捉與脫附白血球的熱敏性聚丙烯纖維薄膜。這個工作製備兩種不同的雙親性共聚物,分別為:N-羥乙基丙烯酰胺-r-甲基丙烯酸丁酯共聚高分子 (poly(HEAA-r-BMA))與N-異丙基丙烯酰-r-甲基丙烯酸丁酯共聚高分子 (poly(NIPAAm-r-BMA))。BMA為疏水性分子鏈段並提供共聚物與聚丙烯纖維薄膜間的分子作用力,以調節共聚高分子於膜表面的穩定性與塗佈量。材料系統選擇HEAA作為捕捉白血球的分子基團而NIPAAm提供薄膜的親水性具有溫度響應功能。透過不同莫爾比HEAA/ BMA與NIPAAm/ BMA (90/10;70/30;50/50;30/70;10/90)來調整共聚高分子中的各項功能,定義出改質聚丙烯纖維薄膜的最佳化的條件,並探討其於白血球捕捉及脫附的性質。 使用HEAA/ BMA為70/30所製備的poly(HEAA70-r-BMA30),改質聚丙烯不織布纖維膜後可呈現高度親水性質。於動態接觸角分析結果顯示可於1分鐘內降至0°,並對於血液中白血球的選擇率高達82%。而使用NIPAAm/ BMA為70/30所製備的poly(NIPAAm70-r-BMA30),改質後的聚丙烯膜在37℃下的水接觸角穩定維持在135°的疏水區間,但在4℃下的水接觸角可於7分鐘內降到0°,且在血液中對白血球的脫附率高達80%。在血液分離過濾實驗中,本研究將改質後的聚丙烯膜進行12層薄膜疊加,並組裝成微型過濾器。在人體全血中的血球分離測試,結果顯示白血球捕捉率可達60%,且低溫白血球回收率近20%。此研究成果指出,使用poly(HEAA-r-BMA)與poly(NIPAAm-r-BMA)改質的聚丙烯纖維薄膜系統,在發展白血球捕捉與脫附的分離技術具高度潛力,並可應用於免疫療法中的淋巴球純化程序。

並列摘要


The research target of this study is to design a thermo-responsive polypropylene (PP) fiber membrane that can be used to capture and detach human leukocytes. This work prepared two different amphiphilic copolymers, namely: poly(N-hydroxyethyl acrylamide-random-Butyl methacrylate) (poly(HEAA-r-BMA)) and poly(N-Isopropylacrylamide-random-Butyl methacrylate) (poly(NIPAAm-r-BMA)). BMA is a hydrophobic segment and provides the molecular interaction between the copolymers and the PP fibers to adjust the stability and coating amount of the copolymer on the membrane surface. The material system chooses HEAA as the molecular group to capture white blood cells, while NIPAAm provides the hydrophilicity of the membrane with temperature responsive function. Through different molar ratios of HEAA/ BMA and NIPAAm/ BMA (90/10; 70/30; 50/50; 30/70; 10/90) to adjust various material functions in the copolymers, which define the modified PP with the optimized conditions and its corresponding properties in capturing and detaching leukocytes. Using poly(HEAA70-r-BMA30) prepared with HEAA/BMA of 70/30, the modified PP non-woven fibrous membrane exhibit highly hydrophilic properties. The dynamic contact angle results show that the 0˚ can be achieved within 1 minute, and the selectivity rate for leukocytes in human blood is as high as 82%. Using poly(NIPAAm70-r-BMA30) prepared with NIPAAm/BMA of 70/30, the water contact angle of the modified PP membranes at 37°C is stably maintained in the hydrophobic range of 135°. The water contact angle can be reduced to 0° within 7 minutes at 4°C, and the desorption rate of leukocytes is as high as 80%. In the blood separation and filtration experiment, this study superimposed 12 layers of modified PP membranes and assembled them into micro-filters. The blood cell separation test in human whole blood showed that the leuko-capture rate at 37°C can reach 60%, and the recovery rate of leukocytes at 4°C is nearly 20%. This study points out that the PP fiber membrane system modified by poly(HEAA-r-BMA) and poly(NIPAAm-r-BMA) has high potential in the separation technology development of leukocyte capture and detachment, and can be applied to lymphocyte purification procedures in immunotherapy.

參考文獻


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