兒茶素,一種普遍的存在於茶、葡萄汁及紅酒中的物質,近年來 的研究指出兒茶素能夠治療癌症,是一種對人體有益的物質。本研究 為結合陰離子掃略式徹底注射(ASEI-Sweeping)技術與微乳化電層析 (MEEKC)技術,發展出陰離子掃略式徹底注射-微乳化電層析 (ASEI-Sweeping-MEEKC)放大技術。此技術克服了過去微乳化電層析 在其他放大技術中因為穩定度不夠而導致放大不佳的瓶頸,並且我們 改變了前端溶液的注射長度及離子濃度,得到不錯的放大效果,另 外,我們還發現了變換微乳化溶液的組成,例如油相及共界面活性劑 的種類及比例,可以得到不同的放大分離效果,並且對於分析物有不 同的選擇性發生,證明了油相種類的不同對於放大效果是有明顯的影 響,此外,我們也改變了樣品的基質,發現在不同的pH 值環境下, 放大的結果有明顯的不同。 最後,由實驗的結果可以發現,使用這個放大的技術能夠有效的 降低偵測極限,另外,將此技術應用在檢測實際樣品上,可以有效偵 測到食品中所含微量兒茶素的種類及含量。
In recent years, particular attentions have been given to catechins because they can prevent from cancer. Since foods (such as tea, grape juice and wine) are sources of a variety of catechins, thus a diet that is good for health. Therefore, the analyses of catechins in foods were very important. In this study, an Anion-Selective Exhaustive Injection-Sweeping MEEKC (ASEI-Sweeping-MEEKC) which was a combined technique of Anion-Selective Exhaustive Injection-Sweeping (ASEI-Sweeping) and MEEKC separation, was successfully established in order to improve the detection limit of catechins for MEEKC method. The length and composition of water plug and high-conductivity solution (HCS) that presented in front of the capillary column were examined for optimizing the ASEI-Sweeping method. In addition, the composition of microemlusion solution (type and concentration of oil and co-surfactant) were also demonstrated as efficient issues in concentration enhancement. Furthermore, the sensitivity enhancement ratio (SER) was markedly influenced by pH values of sample matrix. Finally, the optimal ASEI-Sweeping MEEKC method was successfully used for the analyses of trace catechins in various foods.
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