以射頻電容耦合電漿控制雙離子水凝膠的表面疏水化於皮膚傷口癒合研究

Surface Hydrophobilization of Zwitterionic Hydrogels via RF Capacitively Coupled Plasma for Skin Wound Healing

盧惟中(Wei-Chung Lu)

指導教授 ： 魏大欽

共同指導教授 ： 張雍(Yung Chang)

中原大學/工學院/化學工程研究所/碩士(2021年)

https://doi.org/10.6840/cycu202100442

摘要

本研究利用八氟環丁烷(C4F8)電漿於磺基甜菜鹼(sulfobetaine methacrylate, SBMA)與甲基丙烯酸丁酯(Butyl methacrylate, BMA)共聚之水凝膠高分子進行鍍膜達到表面疏水化，探討不同操作功率下電漿表面鍍膜對水凝膠之透氣率的影響，使傷口能在良好的環境下癒合。並以接觸角量測儀(WCA)、掃描式電子顯微鏡(SEM)、傅立葉轉換紅外線光譜儀(FTIR)、X射線光電子能譜儀(XPS)等儀器分析表面之物理化學性質。
結果發現提升功率能夠提高電漿的活性物種濃度，除了使水凝膠表面鍍上的氟碳膜更完整外，同時也能有效控制敷料之透氣率。在抗生物分子沾黏檢測中發現改質過後的水凝膠仍具有良好的抗蛋白質吸附、細菌、血液細胞貼附能力，藉此可推斷此水凝膠能在不同濕度的環境下進行翻轉，在液相中表面的疏水鏈段會朝向水凝膠內部翻轉，而內部的磺基甜菜鹼分子會朝向外部翻轉，磺基甜菜鹼作為良好的生物相容性材料能有效抵抗生物分子沾黏。藉由這種翻轉的特性能使敷料在傷口端能保有良好的親水性吸取過多的滲液，而在外側能藉由疏水層的保護使傷口能在良好的透氣度下提升傷口癒合效果。由動物實驗中發現傷口癒合的中後階段，經由電漿鍍膜的水凝膠敷料在癒合的效果上會比未經改質的水凝膠敷料來的好，由此可推斷電漿表面鍍膜能有效控制敷料透氣率提升傷口癒合速度。

關鍵字

八氟環丁烷 ； 電漿鍍膜 ； 雙離子 ； 抗沾黏

並列摘要

In this study, octafluorocyclobutane (C4F8) plasma was used for surface hydrophobization on a hydrogel polymer which copolymerized with sulfobetaine methacrylate (SBMA) and butyl methacrylate (BMA). The effect of plasma surface hydrophobization on the air permeability of hydrogel under different operating powers was evaluated so that skin wounds can heal by dressing the hydrogel in a good environment. Water contact angle measuring instrument (WCA), scanning electron microscope (SEM), Fourier transform infrared spectrometer (FTIR), X-ray photoelectron spectrometer (XPS) were to analyze the physical and chemical properties of the plasma-modified surface.
It was found that increasing the plasma power can increase the radical concentration in the plasma, resulting in more fluorocarbon film deposition on the surface of the hydrogel. It can also effectively control the air permeability of the dressing. In the anti-biomolecule adhesion test, it is found that the modified hydrogel still has good resistance to protein adsorption, bacteria, and blood cell adhesion. It can be inferred that the hydrogel can be flipped under different humidity environments. In the liquid phase, the hydrophobic segments on the surface will flip toward the inside of the hydrogel, while the internal sulfobetaine molecules will flip toward the outside. As a good biocompatible material, sulfobetaine can effectively resist the adhesion of biomolecules. With this reversal characteristic, the dressing can maintain good hydrophilicity at the wound end to absorb excessive exudate, and the outer side can be protected by a hydrophobic layer to enhance the wound healing effect under good air permeability. It is found in animal experiments that in the middle and late stages of wound healing, the healing effect of the plasma-coated hydrogel dressing is better than that of the unmodified hydrogel dressing.

並列關鍵字

octafluorocyclobutane ； PECVD ； zwitterionic ； anti-fouling

參考文獻

[1] S. Dhivya, V. Padma, and S. Elango, "Wound dressings – a review," BioMedicine, vol. 5, 11/01 2015, doi: 10.7603/s40681-015-0022-9.

[2] C. Weller, C. Weller, and V. Team, "4 - Interactive dressings and their role in moist wound management," in Advanced Textiles for Wound Care (Second Edition), S. Rajendran Ed.: Woodhead Publishing, 2019, pp. 105-134.

[3] E. Rezvani Ghomi, S. Khalili, S. Nouri Khorasani, R. Esmaeely Neisiany, and S. Ramakrishna, "Wound dressings: Current advances and future directions," Journal of Applied Polymer Science, https://doi.org/10.1002/app.47738 vol. 136, no. 27, p. 47738, 2019/07/15 2019, doi: https://doi.org/10.1002/app.47738.

[4] M. Szycher and S. J. Lee, "Modern Wound Dressings: A Systematic Approach to Wound Healing," Journal of Biomaterials Applications, vol. 7, no. 2, pp. 142-213, 1992/10/01 1992, doi: 10.1177/088532829200700204.

[5] K. Fogh and J. Nielsen, "Clinical utility of foam dressings in wound management: a review," Chronic Wound Care Management and Research, vol. 2015, 02/01 2015, doi: 10.2147/CWCMR.S50832.