含四級胺基之雙離子水凝膠敷料於修復慢性傷口癒合的研究

Study of zwitterionic hydrogel dressing containing quaternary amine groups in repairing chronic wound healing

許雅婷(Ya-Ting Hsu)

指導教授 ： 張雍

中原大學/工學院/化學工程研究所/碩士(2021年)

https://doi.org/10.6840/cycu202100446

摘要

近年來全球患有糖尿病的患者大幅度地增加，糖尿病導致的死亡人數也高居台灣第五名的死因，其中傷口不易癒合是糖尿病最大病徵，因此對於糖尿病傷口治療是近年來備受關注的。本研究提出了具有正電荷的親水性聚合物敷料，應用於二型糖尿病傷口之修復情形。
本研究製備兩種水凝膠系統作為慢性傷口敷料：2-(甲基丙烯酰氧基)乙基三甲基氯化銨(TMA)和聚(乙二醇)甲基丙烯酸甲酯(PEGMA)；2-(甲基丙烯酰氧基)乙基三甲基氯化銨(TMA) 和[2-(甲基丙烯基)乙基]二甲基-(-3-磺酸丙基)氫氧化銨(SBMA)。依據光電子能譜儀 (XPS) 可以確認水凝膠合成是成功的。兩種系統水凝膠表面電荷隨著 TMA 含量增加而增強，同時加強了血小板活化程度、聚集與貼附情形，並能有效的使更高濃度的PDGF-BB與VEGF達到釋放。然而，增加的表面電荷會還是會降低細胞的存活率。應用於糖尿病慢性傷口的評估，TMA、T3S3在第19天時傷口完整癒合，組織病理學判讀為無缺陷、無發炎現象、上表皮化與肉芽組織增生最完整。
因此我們開發一正電結構之水凝膠傷口敷料，依據落於不同區間的表面電位，討論其血小板活化程度、生長因子釋放濃度、細胞毒性以及糖尿病慢性傷口修復之評估間的相關性。

關鍵字

正電材料 ； 水凝膠 ； 血小板活化 ； 生長因子釋放 ； 慢性傷口修復

並列摘要

In recent years, the number of patients suffering from diabetes has increased dramatically in the world. The number of deaths caused by diabetes in Taiwan is also the fifth highest in Taiwan's top ten causes of death. Wounds that are not easy to heal are the biggest symptom of diabetes, so the treatment of diabetic wounds has attracted much attention in recent years.
This study proposed a research system using hydrophilic polymer with different positive charges as a dressing: cationic / electrical hydrogel or cation /zwitterionic hydrogel. We have prepared two hydrogel systems: 2-(methyl-acryloxy) ethyltrimetalammonium chloride(TMA) and poly (ethylene glycol) methyl ether methacrylate(PEGMA)；TMA and sulfoacrylamide (SBMA). From the photoelectron spectrometer (XPS), it can be confirmed that the synthesized hydrogels is successful. The hydrogel surface charge is enhanced as the TMA content is increased while strengthening the activation and aggregation of platelets. And it can effectively release higher concentrations of PDGF-BB and VEGF. Applied to the assessment of chronic diabetic wounds, TMA and T3S3 wounds healed completely on the 19th day, histopathology was no defect, no inflammation, epidermalization and granulation tissue were the most complete.
Therefore, we have successfully developed a hydrogel wound dressing with a positive electrical structure. Based on the surface potentials falling in different intervals, we discussed the correlation between the degree of platelet activation, the release concentration of growth factors, cytotoxicity, and the evaluation of diabetic chronic wound repair.

並列關鍵字

Positive materials ； Hydrogel ； Platelets activation ； Release of growth factors ； Chronic wound repair

參考文獻

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