常壓電漿接枝雙離子與帶正電材料於四氟乙烯薄膜之研究

Modification of Expanded Poly(tetrafluoroethylene) Membranes with zwitterionic and Cationic-polymer Materials by Atmospheric-pressure Plasma

張哲魁(Che-Kuei Chang)

指導教授 ： 魏大欽

中原大學/工學院/化學工程研究所/碩士(2016年)

https://doi.org/10.6840/cycu201600696

摘要

本研究以線性常壓輝光介電質放電(Line Source DBD)對聚四氟乙烯 ePTFE(expanded polytetrafluoroethylene)膜材同時進行表面活化與接枝步驟，接枝甲基丙烯醯氧乙基三甲基氯化銨（TMA）與硫代甜菜鹼（sulfobetaine methacrylate, SBMA）製備出仿生薄膜，其中單體TMA帶正電荷，SBMA帶電中性。經過電漿處理後的膜材利用水接觸角、全反射式傅立葉轉換紅外線光譜儀、掃描式電子顯微鏡、X射線光電子能譜儀等儀器分析表面化學與物理特性，再進行蛋白質吸附及細菌貼附測試，瞭解膜材抗生物沾黏之效果，並於血液實驗中測量改質薄膜之血液相容性。
　　線性常壓輝光介電質放電於固定功率為500 W，掃描次數240次的電漿參數下，製備之PTFE-g-(TMA/SBMA)改質膜材，經由ATR-FTIR與XPS分析所得之正電荷比例瞭解表面電性，當表面呈電中性時最接近仿生薄膜，具有抗生物沾黏效能。生物分子沾黏實驗中，單體配比TMA:SBMA = 0:1且接枝量為1 mg/cm2時表面為電中性，能有效抵抗的蛋白質、血球以及細菌的貼附；而單體配比TMA:SA = 1:1於電漿接枝後，其表面具有吸引血小板之功用，抗人體細胞的沾黏性。
　　本研究所開發的常壓輝光介電質放電可以快速、乾淨、均勻的製備仿生膜材，達到抗生物分子沾黏的性質，其中表面電性為生物分子吸附的原因之一，可運用不同單體電性之混合配比下，製備出所需的表面電性。

關鍵字

常壓電漿 ； 混合電荷 ； 兩性離子 ； 抗生物沾黏膜材

並列摘要

A line source atmospheric dielectric barrier discharge (LSADBD) plasma was used to graft Sulfobetaine (SBMA) and 2-[(methacryloyloxy) ethyl] trimethylammonium chloride (TMA) onto poly(tetrafluoroethylene) (PTFE) membrane. The chemical structure, surface morphology of the surface-modified PTFE membranes were characterized by Fourier Transform Infrared-Attenuated Total Reflectance(FTIR-ATR), Electron Spectroscopy for chemical analysis (ESCA), Scanning Electron Microscopy (SEM), Laser Scanning Confocal Microscope (CLSM). In addition, the anti-biofouling capability of prepared membranes was characterized by the protein adsorption of bovine serum albumin (BSA) and Lysozyme. The anti-attachment ability of the membrane with human blood cell and Escherichia coli was also measured.
　　After modification of PTFE membrane, the grafting density was around 1 mg/cm2 at monomer ratio of TMA : SBMA = 0:1. Since SBMA has both positive-charged （N+(CH3)3） and negative-charged（SO3-）functional groups. The PTFE-g-SBMA membranes possess zwitterionic property which were characterized by SEM, XPS and ATR-FTIR. The zwitterionic surface of membranes not only can prevent protein adsorption, but also can resist the human blood cell and Escherichia coli bacterial attachment. Thus, preparation of zwitterionic materials by line source atmospheric-pressure plasma is successful.
　　The PTFE-g-T1SB1 membrane could effectively resist HT1080 cell adhesion and absorb a large of platelets on the membrane surface. The results indicated the importance of surface charge on the membrane owing to human blood cell and bacterial adhesion.

並列關鍵字

DBD glow discharge ； zwitterionic ； anti-biofouling membrane

參考文獻

[57] 張家仁, "常壓電漿接枝甲基丙烯酸聚乙二醇酯在聚四氟乙烯 薄膜之抗生物沾黏材料運用與探討," 中原大學化學工程研究所學位論文, pp. 1-118, 2009.

[41] 鄭為允, "以高溫微波電漿火炬轉化四氟甲烷與六氟化硫之研究," 私立中原大學碩士論文, pp. 1 - 106, 2007.

[3] 陳彥盛, "由點擊離子配對控制帶正電性殺菌刷狀高分子於細菌可逆釋放之研究," 中原大學化學工程研究所學位論文, pp. 1-84, 2014.

[8] 施曉琳, "常壓電漿接枝類雙離子材料於高分子薄膜之研究," 中原大學化學工程研究所學位論文, pp. 1-144, 2014.

[56] 林文琳, "雙離子與離子混合型共聚高分子結構體之人體血液相容性質," 中原大學化學工程研究所學位論文, pp. 1-114, 2013.