胜?包覆奈米粒子之合成及螢光免疫分析上之應用

許志榮

指導教授 ： 陳家俊

國立臺灣師範大學/理學院/化學系/碩士(2002年)

摘要

無資料

關鍵字

螢光免疫分析 ； 奈米粒子

並列摘要

We utilized the peptide molecules capping ZnS crystal surface to exchange the organic ligand onto CdSe/ZnS core-shell nanoparticle, that resulted in highly fluorescent, water-soluble one. Various sizes of particles via surface functional group exchange could retain their original morphology and optical property such as uv-vis absorption, and fluorescent. To covalently attach the peptide-capped nanoparticles to biological macromolecules, we used cross-linker reagent coupling the nanoparticles and antibodys. The particles-labeled antibodys were employed in fluoroimmunoassays, and the result demonstrated that the particle-antibody conjugation would not affect the activity of protein. These peptide-capped nanoparticles promise to be a versatile tool for probing of biological systems and biolabeling.

並列關鍵字

peptide ； fluoroimmunoassay ； nanoparticle

參考文獻

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