This research mainly uses Visual Basic (VB) to compile the computer’s control interface program, according to the time, frequency and wavelength that the laser acupuncture procedure requires. Started from getting an acupuncture image from the CCD Camera, then uses the image recognition system compiled by VB to recognize the points of the undergoing laser acupuncture treatment, and then uses RS232 to transform the data to microcontroller system, according to different signals this system can control two step motors on the X-Y table to direct points. In the end, uses computer’s control interface program to procedure the laser acupuncture treatment.