微流體系統應用於機械力刺激人體膀胱癌細胞之研究

葉立強(LI-CIANG YE)

指導教授 ： 曹嘉文

國立中央大學/工學院/機械工程學系/碩士(2014年)

摘要

摘要
本研究的目的是設計一個機械力刺激人體膀胱癌細胞的微流體晶片，機械力包括了剪應力與正向應力。微流體晶片上有三條流道，每條流道分別有3個培養區做細胞培養，透過Syringe pump和閥門的控制分別對每條流道施加三種剪應力，再對培養區上方施加正向應力。
在對人體膀胱癌細胞刺激之前，分別對裝置中的機械力做裝置上的測試，剪應力測試使用一般染色液檢測閥門的緊密度，確保每條流道只受到單一剪應力；正向應力測試使用螢光染液觀察培養區螢光強度的變化，用來檢查培養區不會因受到外力而變形。
最後，我們將人體膀胱癌細胞(TCC)培養在微流體晶片的培養區中，分別對細胞施加3組剪應力刺激10分鐘(0.0018 dyne/cm2、0.044 dyne/cm2、0.66 dyne/cm2。)，再對每組施加3組正向應力刺激1個小時(0KPa、10KPa、100KPa)，之後在培養箱中培養24小時，對每組受到不同刺激參數細胞進行拍照，比較刺激前與刺激後的細胞數目。實驗結果顯示受到最大剪應力刺激的細胞，細胞數目有減少的趨勢；0KPa與10KPa正向應力刺激對細胞數量呈現正成長，到了100KPa細胞數目呈現了負成長。

關鍵字

微流體晶片

並列摘要

Abstract
The purpose of this study is to design a mechanical force to stimulate human bladder cancer cell microfluidic chips, mechanical forces, including the shear stress and the normal stress. The microfluidic chip has three channel, each channel has three cultures area were to do in cell culture, three shear stress is applied to each flow path through Syringe pump and control valve, respectively. And then application of normal stress on cultures area.
Human bladder carcinoma (TCC) cultured in the area of the microfluidic chip, the cells were applied to three kinds of shear stress stimulation 10 minutes (0.0018 dyne / cm2,0.044 dyne / cm2,0.66 dyne / cm2.), and then the each of three shear stress applied normal stress stimulation one hour (0KPa, 10KPa, 100KPa), then cultured in an incubator for 24 hours, subject to different stimulation parameters for each group cells were photographed, the number of stimulation cells before and after.
Experimental results show that by the cell, the cell number of the maximum shear stress stimulation downward trend; 0KPa and 10KPa normal stress stimulation on the number showed positive growth, the number of cells to 100KPa presented negative growth.

參考文獻

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