鯉魚表皮生長因子的選殖及特性分析，及其於不同組織及早期發育階段的表現

表皮生長因子(EGF）被認為在生殖系統中具有生長因子的功能。其中一項功能是加快卵母細胞的成熟。本研究的目的是探討EGF在鯉魚(Cyprinus carpio)各組織及早期發育階段（卵及幼魚）中的表達特徵。表皮生長因子（EGF）的cDNA透過反轉錄聚合酶鏈反應從鯉魚卵巢中選殖出，並確認所獲得的序列。所獲得的序列比對斑馬魚的同源序列達到81.5％的相似性。然而，遺憾的是本研究尚未發現鯉魚EGF的全長基因，因此在進一步的實驗中只使用部分序列。鯉魚不同組織中EGF的表達透過即時PCR進行測定。結果顯示，幾乎所有的測試組織中EGF濃度不具有顯著差異。而觀察卵巢發育階段的EGF表達使用注射HCG的鯉魚卵巢及其受精卵與幼魚進行。所有的組別皆有類似的結果，並無顯著差異。造成此結果的原因可能是EGF來自EGF家族且具有較長的序列。然而，在本研究中只使用部分序列，使引子不具有特異性，使數據中顯示的EGF可能來自一種以上的鯉魚EGF。

關鍵字

表皮生長因子（EGF）；生殖；鯉魚

並列摘要

Epidermal growth factor (EGF) is a growth factor that is thought to have a function in the reproduction system. One of them is accelerating the maturity of oocyte. The purpose of this research is to investigate characterization of EGF in carp (Cyprinus carpio) and determine the expression of EGF in various tissues and the early stages of development on fish (eggs to larvae). Epidermal growth factor (EGF) cDNA was cloned from ovary by a reverse transcription polymerase chain reaction and confirm to get sequence. The similarity of homologous sequences of 81.5% against the zebra fish. However, unfortunately on this research still not found full length on carp EGF, so at next treatment of the test expression using only partial sequences. Different tissues on carp, were tested using real time PCR to quantify the expression of EGF. The results showed, almost all of the tissues to be tested did not have a significant difference. Other treatment conducted by observing the EGF expression in ovarian development stage which had been injected with HCG, the using of egg and larval stages of development. Similar results are shown on all treatments, there is no significant difference on any observations. One possible reason might be EGF has a long sequence and from the EGF family. Whereas, in this study, only get partial sequences, so that the primer might be not specific and the expression shown in the data may be derived from more than one EGF on carp.

並列關鍵字

Epidermal growth factor (EGF) ； Reproduction ； Carp

參考文獻

Afraei Bandpei, M.A., 2011. The Environmental Effect on Spawning Time, Length at Maturity and Fecundity of Kutum (R u t i l u s f r i s i i k u t u m , Kamensky, 1901) in Southern Part of Caspian Sea, Iran. Iran. J. Energy Environ.

Ben-Ami, I., Armon, L., Freimann, S., Strassburger, D., Ron-El, R., Amsterdam, A., 2009. EGF-like growth factors as LH mediators in the human corpus luteum. Hum. Reprod. 24, 176–184.

Carnevali, O., Cionna, C., Tosti, L., Lubzens, E., Maradonna, F., 2006. Role of cathepsins in ovarian follicle growth and maturation. Gen. Comp. Endocrinol. 146, 195–203.

Carpenter, G., 1985. Epidermal growth factor: biology and receptor metabolism. J. Cell Sci. Suppl. 3, 1–9.

Carpenter, G., Cohen, S., 1990. Epidermal growth factor. J. Biol. Chem. 265, 7709–7712.

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鯉魚表皮生長因子的選殖及特性分析，及其於不同組織及早期發育階段的表現

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