探討不同病毒感染複數對Ad-DR bioassay準確性之影響

Ad-DR bioassay檢測技術已趨於成熟與穩定，許多的文獻均證實該方法有足夠的穩定度與敏感度可扮演戴奧辛生物快篩的角色，先經由生物分析法篩選樣品，挑出污染樣品進行化學分析，降低化學分析方法的時間與成本。台灣地區目前仍以化學分析法做為戴奧辛檢測之標準方法，故民間檢測單位大部份是以化學背景的專業人士為主，導致在推廣Ad-DR bioassay時，生物技術的操作成為一種必須重新學習的門檻。為了增加Ad-DR bioassay的操作便利性，與靈敏度和穩定度，本次研究將探討不同病毒感染複數(MOI)對提高Ad-DR bioassay的靈敏度與穩定性是否有影響，尋找更符合現況的應用面。本次研究將使用化學與生物分析全台北部、中部、南部、東部、離島等區域共160個土壤樣品。其中93個土壤與67個樣本分別使用MOI=2與MOI=10之Ad-DR bioassay進行檢測，並與化學分析法數據進行比較。本次研究顯示低濃度樣品中(< 5 ng I-TEQ/kg )比較Ad-DR bioassay與化學法的比值可發現，MOI=10與化學法的平均比值(mean: 6.93)較MOI=2平均比值(mean: 9.21)來的低，特別是在濃度低於1 ng I-TEQ/kg以下之土壤樣品，顯示MOI=10檢測能力優於MOI=2。在穩定度方面MOI=2與MOI=10之數據並無顯著的差異，但MOI=10之實驗數據冷光讀值較高，其RSD均容易符合品質管制標準，較適合業者使用與推廣。

關鍵字

戴奧辛；感染複數；土壤

並列摘要

Several studies demonstrate that Ad-DR bioassay is a stable and sensitive method for screen dioxin levels in environment and biota samples. Recent techniques for quantification of dioxins and dioxin-like compounds involve mainly the costly and time-consuming high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) in Taiwan. Due to most analysts are major in chemical analysis which is a bottleneck of promotion Ad-DR bioassay. Therefore, the aim of this study is use different multiplicity of infection (MOI) to inprove the sencitive and stable ability of AD-DR bioassay. One hurndred sixty dust samples were collected from Northern, Central, Southern and East Taiwan. Ninety-three and sixty-seven dust samples were tested by two kinds conditions of Ad-DR bioassay (MOI=2 and MOI=10), respectively, and further to compared with chemical analysis. In the present study, the mean ratio (bioassay/chemical) of MOI=10 was 6.93 fold better than MOI=2 (mean: 9.21 fold) in low concentration soil sample (< 5 ng I-TEQ/kg), especially in extreme low concentration soil sample (< 1 ng I-TEQ/kg). Although the relative standard deviation (RSD) between MOI=10 and MOI=2 did not observed significant different, MOI=10 of Ad-DR bioassay is more suitable for promotion Ad-DR bioassay to commercial laboratory.

並列關鍵字

Dioxin ； Ad-DR bioassay ； multiplicity of infection ； soil ； Taiwan

參考文獻

李俊賢、詹長權、王榮德，從戴奧辛毒性談台灣醫療事業廢棄物焚化處理，中華衛誌，Vol.18，pp.237-240，1999。

許菁芳，2000。油症患者血液中多氯聯苯、多氯戴奧辛、多氯夫喃同源物濃度與肝功能異常之相關性研究，國立成功大學，環境醫學研究所，碩士論文。

Bhavsar SP, Reiner EJ, Hayton A, Fletcher R, MacPherson K, 2008. Converting Toxic Equivalents (TEQ) of dioxins and dioxin-like compounds in fish from one Toxic Equivalency Factor (TEF) scheme to another. Environment International 34(7): 915-921.

Chao, H.R., Wang, Y.F., Lin, D.Y., Cheng, Y.T., Tsou, T.C., 2011b. Fast cleanup system combined with adioxin-responsive element-driven luciferase bioassay for analysis of polychlorinated dibenzo-p-dioxins/furans in sediments and soils. Bull Environ Contam Toxicol. 86:278-282.

De Mul A, Bakker MI, Zeilmaker MJ, Traag WA, Leeuwen SP, Hoogenboom RL, Boon PE, Klaveren JD, 2008. Dietary exposure to dioxins and dioxin-like PCBs in The Netherlands anno 2004. Regul Toxicol Pharmacol 51(3), 278-287.

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探討不同病毒感染複數對Ad-DR bioassay準確性之影響

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