結合耐鎘細菌之接種與施用螯合劑以增強鎘污染土壤之植生萃取效率

植生復育(phytoremediation)是近年來整治重金屬污染土壤，較為常用的一種生物處理整治技術，其優點是整治過後之土壤，還可保有原本之性質，且較不易對環境產生影響，是一種對環境非常友善之整治技術，且成本也很低廉。不過其缺點是整治時間較長，土壤中生物量較少，導致其植體較不易吸收。添加螯合劑於土壤中，可提高重金屬之溶解度，使植體的吸收量增加，而接種菌種可以改善土壤之環境，增加植體的吸收量。因此，本研究結合添加菌種至土壤及添加2種螯合劑(EDDS及NTA)來增強植生萃取之效果，以強酸性紅壤添加不同濃度鎘(2.5、5.0mg/kg)後，種植空心菜(Ipomoea aquatic Forsk)以評估植生萃取效果。實驗結果顯示，添加螯合劑與菌種並沒有使植體的產量增加，但是卻有使土壤中之菌數增加，其中以添加Cd-01及EDDS的組別增加最多菌數，由第7天2.3×107增加至第28天的7.0×107。添加2.5及5 mmol/kg NTA及Cd-01為空心菜植生萃取鎘最佳的組合處理，其生物累積因子，不管是在鎘2.5 mg/kg及5.0 mg/kg的污染土當中均都高於空白組。這說明了添加螯合劑與菌種有助於植體累積重金屬。

關鍵字

乙二胺二琥珀酸(EDDS) ；三乙酸基氨(NTA) ；鎘；植生復育；空心菜；接種

並列摘要

Phytoremediation is commonly used biotechnique that it repairs heavy metal pollution soil in recent years. The advantage of maintain the original nature and less likely to impact on the environment after the soil remediation, but also to is very friendly and low cost of remediation environmentally technology.But the disadvantage that it have longer remediation time andless biomass in soil, leading to implant more difficult to absorb.The results show the soil adding chelating agents can increase the solubility of heavy metals so that it raise the absorption of implants. Other studies show that the vaccine strains can improve the soil environment, increase the absorption of implants. Therefore, inoculated of EDDS and NTA respectively were spiked into Cd (2.5、5.0mg/kg) after planting water spinach (Ipomoea aquatic Forsk) in order to assess phytoextraction efficiency. The results show that adding chelating agents and bacteria did not increase the production of implants, but there's bacteria in the soil increase the number of which add Cd-01 and groups of EDDS increased the most, by the 7th day 2.3 × 107 increased to 28 days more than 7 × 107 nearly three times more than the control group, but also nearly four times as many, illustrates the added chelating agent and the soil bacteria will increase the number of bacteria. Add 2.5 and 5 mmol / kg NTA and add Cd-01 greens of vegetation for the best combination of cadmium extraction process, the bioaccumulation factor, whether in the Cd 2.5 mg / kg and 5.0 mg / kg of contaminated soil were higher than among control group. This shows that adding chelating agents to help with the bacteria accumulate heavy metals implants.

並列關鍵字

[S,S]-ethylenediaminedisuccinic acid ； nitrilotriacetic acid ； Cadmium ； Phytoextraction ； Ipomoea aquatica Forsk ； inoculated

參考文獻

賴鴻浴，2004，EDTA對促進重金屬鎘鋅及鉛污染土壤植生復育之研究，國立台灣大學環境工程研究所博士論文，台北市，第8-12頁。

Alloway B. J., 1995, “Heavy metals in soils.”Blackie Academic &Professional, Glasgow, UK.

Araujo J. do C. T. de, and Nascimento, C. W. A. do, 2009, “Phytoextraction of Lead from Soil from a Battery Recycling Site: The Use of Citric Acid and NTA,” Water, Air, Soil Pollut., DOI 10.1007/s11270-009-0285-4.

Baker A. J. M., McGrath, S.P., Reeves, R. D., Smith, J. A. C., 2000, “Metal hyperaccumulator plants: a review of the ecology and physiology of a biological resource for phytoremediation of metal-polluted soils. In: Terry, N., Bañuelos, G. (Eds.), Phytoremediation of Contaminated Soil and Water.” Lewis Publisher, Boca Raton, Florida, pp. 85–107

Belimov A. A.,Hontzeas N.,Safronova V. I., Demchinskaya S. V., Piluzza G.,Bullitta S.,Glick B. R., 2005, “Cadmium-tolerant plant growth-promoting bacteria associatedwith the roots of Indian mustard (Brassica juncea L. Czern.) ” Soil Biology & Biochemistry (37)241–250.

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結合耐鎘細菌之接種與施用螯合劑以增強鎘污染土壤之植生萃取效率

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