產業放流水溶解性有機質光學特性

Dissolved Organic Matter (DOM) Optical Property in Industrial Effluents

林明賢(Ming-hsien Lin)

指導教授 ： 陳庭堅

國立屏東科技大學/工學院/環境工程與科學系所/碩士(2012年)

https://doi.org/10.6346/NPUST.2012.00247

摘要

溶解性有機物質(DOM)的來源廣泛且具多樣性，會影響有機污染物之分配、吸附、傳輸及生物毒性，扮演生物化學循環的關鍵角色。螢光光譜是很敏銳的技術，應用於追蹤海洋與淡水中自然與人為的溶解有機質的動態。本研究分析鋼鐵廠(P1)、面板廠(P2)、樹脂廠(P3)、化纖廠(P4)及一般生活污水處理廠(P5)放流水，以紫外線/可見光分光光度計量測各樣本吸光值，螢光光譜儀檢測螢光激發-放射矩陣光譜(EEM)，除觀察各樣本之螢光光譜特徵外，計算UV指數、螢光指數及螢光區域積分(FRI)，並計算各指數相關性，探討光學指數對於不同工業廢水之適用性。研究結果顯示以波長254 nm時的吸光值除以DOC濃度(SUVA254)作為判斷分子大小及腐植化程度的工具適用於P1、P2、P4、P5。各樣本螢光光譜圖形越相似，區域積分佔百分比也越相近，顯示以區域積分法判讀螢光光譜圖是適當的方法。比較原液、截留液及滲濾液的螢光指標發現P1、P2、P4及P5之DOM為微生物或水體細菌產生的新生DOM，P3為非微生物性的其他DOM。以UV/vis指數判斷，波長250 nm和360 nm時吸光值的比值(E2/E3)為負值時，和DOM分子量大小成反比的關係並不存在。對於放流水中DOM種類之判斷，綜合波峰加上區域積分的結果應是較為完整的判斷方式。S275於P1、P2、P4、P5之比對結果，對於判斷DOM分子大小與Helms等(2008)的研究結果一致。綜合三種濾液分析之結果，以SUVA254為基礎，發現波長250 nm-450 nm吸光值的總和(A250)在三種濾液中呈現顯著的正相關性，是適合應用在判斷工業放流水分子大小的指標。SUVA254與生物性指標(BIX)在原液及滲濾液中呈現顯著的正相關性，推斷在原液及滲濾液中微生物來源的DOM越高呈現出越高的腐植化程度。

關鍵字

溶解性有機質 ； 紫外光/可見光指標 ； 螢光指標

並列摘要

Dissolved organic matter (DOM) originates from a variety of sources, impacts distribution, absorption, transport, and biotoxicity of organic pollutants, and plays an important role in biochemical cycles in an aquatic environment. Fluorescence spectroscopy is a sensitive technique, which is used to track the movement of natural and synthetic DOM in ocean and freshwater. The wastewater effluent DOMs (EfOM) of a steel plant (P1), a panel plant (P2), a resin plant (P3), a chemical fiber plant (P4), and a sewage treatment plant (P5) were analyzed in this study with UV/vis and fluorescence spectra to detect absorbance and excitation-emission matrix (EEM) of EfOM samples. The UV/vis and fluorescence spectral indices, percentage of fluorescence region integration (FRI), and correlations of each index were examined in order to find out applicability of an optical index for the different EfOMs. The results show specific ultraviolet absorbance at 254 nm divided DOM carbon concentration (SUVA254), used as a tool to determine molecular size and extent of humification, are applicable to EfOM of P1, P2, P4 and P5. The similar fluorescence spectra of each EfOM sample had closer FRI percentage, which suggests FRI is a suitable way to analyze fluorescence spectra. In this study, EfOMs of P1, P2, P4, and P5 are similar in chemical composition and structure of DOMs generated by microbial or bacterial in water; however, the EfOM of P3 consisted of non-microbial DOM when compared to fluorescent indices of raw, retentrate, and permetate solutions. Considering UV/vis index, the values of ratio of absorbance at 250 nm and 365 nm (E2/E3) were negative and the inverse proportion to DOM molecular weight did not exist. Regarding the distinguishing type of DOM in EfOMs, the integrated peak location with its regional integration percentage is a more complete judgment. The comparison values of the slope of the 275-295 nm absorbance region (S275), the way to determine DOM molecule size in EfOM of P1, P2, P4, and P5, were consistent with Helms’s (2008) study results.
Based on value of SUVA254, the index of absorbance integral area from 250 nm to 450 nm (A250) in three kind solutions shows significantly positive correlations with values of SUVA254 that is applicable in determining molecule size and hmuification of industrial EfOM. The biological index (BIX) values in original and penetrate solutions showed significant positive correlation with SUVA254.

並列關鍵字

DOM ； UV/Vis index ； fluorescence index

參考文獻

周明玄，2011，鴛鴦湖湖水溶解性有機碳之來源探討，碩士論文，國立台灣大學，生態學與演化生物學研究所，台北。

廖憶華，2006，以光學頻譜分析定性及定量廢水水質特性之研究，碩士論文，國立中央大學，環境工程研究所，桃園。

賴文亮，2002，各種淨水程序對生物可分解有機質及消毒副產物之控制，博士論文，國立成功大學，環境工程研究所，台南。

A. Huguet, L. Vacher, S. Relexans, S. Saubusse, J.M. Froidefond, E. Parlanti, 2009, “Properties of fluorescent dissolved organic matter in the Gironde Estuary,” Organic Geochemistry, Vol. 40, pp. 706-719.

Baker, A., 2001, “Fluorescence excitation-emission matrix characterization of some sewage-impacted rivers,” Environmental science & technology, Vol 35, pp. 948-953.