穀物飼料中的赭麴毒素A之定量分析及除污技術開發

Quantitative analysis and decontamination method for ochratoxin A in cereal feed

王凱平(Kai-Ping Wang)

指導教授 ： 錢偉鈞

朝陽科技大學/理工學院/應用化學系/碩士(2015年)

摘要

赭麴毒素A (Ochratoxin A，OTA)由真菌中麴黴菌屬(Aspergillus sp.) 和青黴菌屬(Penicillium sp.) 菌株產生的次級代謝產物，廣泛污染糧食和飼料。赭麴毒素A具有很強的腎臟和肝臟毒性，並有致畸、致突變、免疫抑制和致癌等作用，對動物和人體健康有很大的潛在危害。本研究利用免疫親合管柱結合液相層析串聯式質譜儀，針對自2012年7月至2014年9月間亞洲八個地區229件之穀物及飼料樣品中之赭麴毒素A進行定量檢測，結果顯示平均含量為5 ng/mL，最高含量96 ng/mL，總檢出率達31 %。根據此文進一步分析區域污染分佈及赭麴毒素A與其他黴菌毒素共發性。本研究利用蒙托土及羅勒籽和奇亞籽作為赭麴毒素A吸附除污之資材，體外模擬禽畜動物腸胃酸性環境下比較二者吸附赭麴毒素A之吸附效能。結果顯示，當蒙托土與赭麴毒素A重量比超過3105其吸附成效可達90%，羅勒籽和奇亞籽重量比必需超過6106吸附率方能達90%。據此結果將討論二項天然資材進行毒素吸附之機制。

關鍵字

蒙托土 ； 液相層析串聯式質譜儀 ； 赭麴毒素A ； 羅勒籽 ； 奇亞籽

並列摘要

Ochratoxin A (OTA) is a mycotoxin mainly produced as a secondary metabolite by fungi of the Aspergillus (Aspergillus sp.) and Penicillium (Penicillium sp.) species. It contaminates food and feed and has strong kidney and liver toxicity. It's teratogenic, mutagenic, and immunosuppressive and carcinogenic effects are potential hazards to animal and human health. In this study, we applied by immunoaffinity column integrated with high performance liquid chromatography tandem mass (LC/MS/MS) to analyse. The OTA quantitatively in 229 cereal and feed samples, which are collected from eight regions of Asia during July 2012 to September 2014. The results showed that the average level of OTA in cereal samples was 5 ng/mL, the maximum value was 96 ng/mL, and the occurrence is 31%. The difference of OTA contamination level among these regions and the co-occurrence of OTA with other toxins further discussed. Meanwhile we used montmorillonite and chia seed and basil seed as decontamination materials. The efficiency of OTA adsorption by montmorillonite and chia seed and basil seed was compared under the environment, simulate the livestock animal stomach for acidity in vitro. The results showed that when the weight ratio of montmorillonite to OTA over 3x105, decontamination efficiency achieved 90%; however, the weight ration of basil seed and chia seed to OTA needs to be over 6x106 to approach 90% adsorption of OTA. Based on the results, the mechanism of toxin adsorption by these two decontamination materials is discussed.

並列關鍵字

Basil Seed ； Montmorillonite ； LC/MS/MS ； Ochratoxin A ； Chia Seed

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