Pseudomonas aeruginosa K-187發酵蝦蟹殼於空心菜青枯病防治之研究

The Studies on the Antimicrobial Materials of Jiaosi Ipomoea aquatica Forsk Bacterial Wilt

王全祿(Chuan-Lu Wang) ； 黃詩堯(Shih-Yao Huang) ； 吳建緯(Jian-Wei Wu) ； 劉朋衢(Pon-Cheu Liu)

《蘭陽學報》 7期 (2008/06) Pp. 17-22

https://doi.org/10.6665/JLYIT.2008.7.17

摘要

近年來，礁溪溫泉空心菜因感染青枯病(Ralstonia solanacearum)而產量大減，原本全鄉一年約可生產一千多公噸，現在約只剩下四百多公噸。受到青枯病影響產量，投入種植的農民也越來越少，相關單位亦積極在研究有效處理方法。過去在農作物病蟲害管理上，幾乎都仰賴化學農藥。然而，化學農藥具有價格貴、易造成環境污染以及過量使用產生抗藥性菌系等缺點。由於目前仍無根除方法，農民僅能靠輪作、土壤改良和採收前降低水位等方式降低損失。
本研究之主要目的係以Pseudomonas aeruginosa K-187菌株發酵蝦蟹殼水產廢棄物，生產青枯病原菌(Ralstonia solanacearum)抑制物，探討有否助於抑制青枯病及助礁溪溫泉空心菜生長為研究目的。最適生產條件為：2%蝦蟹殼粉、0.1%K2HPO4、0.05%MgSO4•7H2O之培養基，於pH7、37℃振盪培養4天，可得最大抑制活性。

關鍵字

蝦蟹殼粉 ； 青枯病 ； 生物製劑

並列摘要

Recently the bacterial wilt of Jiaosi Ipomoea aquatica Forsk caused by Ralstonia solanacearum occurred. Because the R. solnacearum infection is the main cause of dead Jiaosi Ipomoea aquatica Forsk, this research will study the utilization of shrimp shell wastes by microorganism to produce antimicrobial agent and the effect of such an agent on the Jiaosi Ipomoea aquatica Forsk culture.
The experiment will perform in two parts. First step: Studies on the antimicrobial materials produced by P. aeruginosa K-187. Second step: Field experiment. Maximum inhibitory activity was obtained when P. aeruginosa K-187 was grown aerobically in a medium consisting 2% shrimp and crab shell powder, 0.1%K2HPO4 and 0.05%MgSO4•7H2O at a pH of 7.0.

並列關鍵字

shrimp and crab shell powder ； Ralstonia solnacearum ； antimicrobial materials

參考文獻

Chen, H. C.,Hsu M. F.,Jiang S. T.(1997).Purification and characterization of an exo-N, N'-diacetylchitobiohydrolase-like enzyme from Cellulomonas flavigena NTOU 1.Enzyme Microb Technol.20,191-197.

Evvyernie D.,Morimoto K.,Karita S.,Kimura T.,Sakka K.,Ohmiya K.(2001).Conversion of chitinous wastes to hydrogen gas by Clostridium paraputrificum M-21.J Biosci Bioeng.91,339-343.

He H.,Chen X.,Sun C.,Zhang Y.,Gao P.(2006).Preparation and functional evaluation of oligopeptide-enriched hydrolysate from shrimp (Acetes chinensis) treated with crude protease fromBacillus sp. SM98011.Biores Technol.97,385-390.

Li S.J.,Seymour T.A.,King A.J.,Morrissey M.T.(1998).Color stability and lipid oxidation of rockfish as affected by antioxidant from shrimp shell waste.J Food Sci.63,438-441.

Teng W.L.,Khor E.,Tan T.K.,Lim L.Y.,Tan S.C.(2001).Concurrent production of chitin from shrimp shells and fungi.Carbohyd Res.332,305-316.