The purpose of this study was to explore the influence of store impression, professional ability, and satisfaction of chain pharmacies on customer loyalty. A purposive sampling method was adopted to distribute the questionnaires; a total of 320 questionnaires were distributed, and 300 were collected, with a return rate of 93.7%. After excluding 25 invalid questionnaires, there were 275 valid questionnaires, with an effective recovery rate of 91.6%. The questionnaires were analyzed by SPSS and AMOS. The results indicate that: 1) Store impression had a significant influence on customer loyalty. 2) Professional ability had no significant influence on customer loyalty. 3) Satisfaction had a significant influence on customer loyalty. Finally, according to the study results, some suggestions on the practical application were provided.