Potato and Tomato Forever Young Genes Contain class-I Patatin promoter-like Sequences

馬鈴薯及蕃茄Forever Young基因含有類似class-I patatin基因啓動子之序列

符宏勇(Hong-Yong Fu) ； 杜杰(Ji Du) ； 宋俊岐(Jun-Qi Song) ； 蔣繼明(Ji-Ming Jiang) ； William D. Park

《Botanical Bulletin of Academia Sinica》 42卷4期 (2001/10) Pp. 231-241

https://doi.org/10.7016/BBAS.200110.0231

摘要

馬鈴薯塊莖主要儲藏蛋白patatin之class-I基因，其近端啓動子所含重複序列爲該基因受發育及代謝調控之重要cis-elements.先前限制酵素片斷多型性分析(RFLP)顯示馬鈴署及蕃茄之patatin基因位於相似之基因座，即靠近第八條染色體長臂之末端。與圖定位顯示馬鈴薯與蕃茄之第三條染色體中央含有與patatin基因近端啓動子重複序列極同源之基因座[Ganal et al, Mol.Gen.Genet.225, 501-509(1991)]。我們自蕃茄、栽培種馬鈴薯(S.tuberosum)與野生種馬鈴薯(S.bulbocastanum)將此類似class-I patatin啓動子序列(PPL代表class-I Patatin Promoter-Like sequences)分離出來。序列比較顯示PPL含有與patatin基因上重要cis-elements極同源之序列。PPL位於Forever Young基因轉譯起始點之上游，該基因轉譯出之蛋白屬於一含保守結構之氧化還原酵素群。蕃茄與S.bulbocastanum之Forever Young基因皆含有八個exons與七個introns,其中六個introns皆格外的大，介於418~2,092bp之間。序列之高度保守性指出class-I patatin基因之重複序列與Forever Young基因之PPL可能有直接之演化親緣關係，並可能透過類似之cis-及trans-因子來調控其相對應基因之表逹。

關鍵字

Forever Young ； 氧化還原酵素 ； patatin ； 馬鈴薯 ； 啓動子演化 ； 蕃茄

並列摘要

Class-I patatin genes from potato, encoding the major storage protein in tubers, contain promoter repeat sequences, which are critical for developmental and metabolic regulation. Previously, RFLP mapping indicated the patatin genes of potato and tomato reside in orthologous loci near the end of the long arm of chromosome VIII. Interestingly, a DNA sequence homologous to the promoter of the potato class-I patatin gene was mapped to the middle of chromosome III in the tomato and potato genomes [Ganal et al., Mol. Gen. Genet. 225, 501-509 (1991)]. We isolated and characterized genomic clones containing the class-I Patatin Promoter-Like sequences (PPL) from tomato, S. bulbocastanum, and S. tuberosum. Sequence analyses reveal that these PPL sequences are highly conserved, as compared to the repeat sequence of the patatin promoter, particularly with respect to the critical regulatory ciselements. These PPL sequences are located upstream of the translational initiation site of Forever Young genes, which encode proteins belonging to a large family of oxidoreductases with conserved structural motifs. The Forever Young genes from tomato and S. bulbocastanuin both contain eight exons and seven introns. Six of the introns are uniquely large, in the range of 418—2,092 bp. Sequence conservation suggests that the class-I patatin repeat and the PPL from Forever Young genes may have a direct phylogenetic relationship and may regulate expression of their respective genes through common cis- and trans-factors.

並列關鍵字

Forever Young ； Oxidoreductase ； Patatin ； Potato ； Promoter evolution ； Tomato