Two new species of the genus Trichodina Ehrenberg, 1838 were obtained during surveys of trichodinid ciliophorans in the Churni River system and adjacent water bodies in West Bengal, India. These are T. giurusi sp. n. from Glossogobius giuris (Hamilton-Buchanan) and T. molae sp. n. from Amblypharyngodon mola (Hamilton-Buchanan). This papers deals with taxonomic descriptions of the two new species based on wet silver nitrate impregnated specimens along with prevalence and morphometric comparisons with closely related species.