Title

Molecular Cloning and Functional Characterization of Protein Phosphatase 2C of Two Scuticociliates-Uronema marinum and Miamiensis avidus (Ciliophora: Scuticociliatia)

Authors

Eun Hye Lee;Ki Hong Kim

Key Words

Protein phosphatase 2C PP2C ; Scuticociliates ; Uronema marinum ; Miamiensis avidus

PublicationName

Acta Protozoologica

Volume or Term/Year and Month of Publication

49卷4期(2010 / 12 / 01)

Page #

281 - 288

Content Language

英文

English Abstract

Complementary DNAs (cDNAs) of protein phosphatase 2C (PP2C) were cloned from two marine scuticociliates Uronema marinum and Miamiensis avidus. Both PP2C proteins showed structural characteristics of typical PP2C, such as highly conserved amino acid residues predicted for binding to phosphate and metal ions, 11 conserved PP2C motifs and 10 invariant residues. The phosphatase activity of recombinantly produced U. marinum PP2C (UmPP2C) was in proportion to the PP2C protein and Mg(superscript 2+) concentrations, and was not sensitive to okadaic acid, but was inhibited by sodium fluoride, EDTA or Ca(superscript 2+). The expression of UmPP2C was significantly up-regulated by exposure the ciliates with PMA suggesting that UmPP2C dephosphrylates proteins phosphorylated by protein kinases as in other eukaryotes and has a regulatory function against abrupt increase of protein phosphorylation triggered by strong stimulations.

Topic Category 生物農學 > 生物科學