Title

冠瘤海鞘幼体附着和变态的离子控制

Translated Titles

Ionic Control of Settlement and Metamorphosis in the Tadpole Larvae of Ascidian, Styela canopus Savigny

Authors

黄英(Ying Huang);柯才焕(Cai-Huan Ke);冯丹青(Dan-Qing Feng);周时强(Shi-Qiang Zhou);李复雪(Fu-Xue Li)

Key Words

冠瘤海鞘 ; 幼体 ; 附着 ; 变态 ; 离子控制 ; 海鞘 ; Styela canopus Savigny ; larvae ; settlement ; metamorphosis ; ionic control ; ascidian

PublicationName

海洋學報

Volume or Term/Year and Month of Publication

27卷1期(2005 / 01 / 01)

Page #

137 - 144

Content Language

簡體中文

Chinese Abstract

研究了人工海水中三种金属离子K(上标 +),Mg(上标 2+)和Ca(上标 2+)分别在不同浓度下对冠瘤海鞘(Styela canopus Savigny)幼体附着和交态的影响,同时采用K(上标 +)通道阻滞剂TEA检验其可兴奋细胞膜上的K(上标 +)通道特性,对冠瘤海鞘幼体附着和变态的离子控制机制进行初步探讨,结果表明:K(上标 +)的浓度为20~40mmol/dm^3时诱导幼体附着和变态的效果最佳,低于4.5mmol/dm^3和高于80mmol/dm^3的K(上标 +)浓度都表现出抑制效果;Mg(上标 2+)浓度在0~32mmol/dm^3时显着促进幼体附着和变态,达88mmol/dm^3时起抑制作用;在人工海水中无论添加或减少Ca(上标 2+)均有效抑制幼体变态。TEA不能阻抑增加K(上标 +)的诱导作用,这表明冠瘤海鞘幼体可兴奋细胞膜上的K(上标 +)通道对外部TEA不敏感。另外,人工海水中缺乏K(上标 +)或Ca(上标 2+)或K(上标 +)浓度达80mmol/dm^3都会对冠瘤海鞘幼体产生毒害作用。

English Abstract

The effects of changes in concentrations of potassium, magnesium and calcium on settlement and metamorphosis in the Styela canopus Savigny larvae in artifical seawater and the characteristic of the potassium channels in externally accessible, excitable cell membrane were studied. K(superscript +) effectively induced larval settlement and metamorphosis during the concentrations of 20~40mmol/dm^3, but showed inhibitive effect in the concentrations being less than or egual to 4.5mmol/dm^3 or being greater than or egual to 80mmol/dm^3. The inductive effect of 0~32mmol/dm^3 Mg(superscript 2+) was remarkable, but 88mmol/dm^3 Mg(superscript 2+) inhibited larval settlement and metamorphosis. The settlement and metamorphosis of the larvae would be inhibited no matter whether increased or decreased Ca(superscript 2+) in artificial seawater. The failure of inhibiting the inductive effect of K(superscript +) by a K(superscript +)-channel blocker TEA (tetraethylammonium chloride) indicated that the potassium channels in externally accessible, excitable cell membrane of Styela Canopus Savigny larvae are insensitive to external TEA. Like 80mmol/dm^3 K(superscript +), K(superscript +)-free or Mg(superscript +)-free seawater would produce evidence of toxicity. The mechanisms of ionic control of settlement and metamorphosis in the Styela canopus Savigny larvae were discussed.

Topic Category 基礎與應用科學 > 海洋科學