Title

球形α-殼聚糖固定化糖化酶的比較研究

Translated Titles

Comparative Research on Immobilization of Glucoamylase with Globe α-chitosan

Authors

張劍(Jian Zhang);王又容(You-Rong Wang);陳進(Jin Chen);羅瑜(Yu Luo)

Key Words

球形α-殼聚糖 ; 脫乙酰度 ; 固定化酶 ; 糖化酶 ; globle α-chitosan ; degree of deacetylation ; immobilized enzyme ; glucoamylase

PublicationName

中國釀造

Volume or Term/Year and Month of Publication

2005卷12期(2005 / 12 / 20)

Page #

15 - 18

Content Language

簡體中文

Chinese Abstract

α-甲殼素經高濃度NaOH溶液處理後,製成了3種不同脫乙酰度的殼聚糖載體,用戊二醛接枝,與游離酶偶聯後便得到固定化酶。固定化酶性質和動力學參數的研究表明,脫乙酰度高的殼聚糖載體固定化糖化酶的性能最好:其酶回收率達75%;最適反應pH6.0~7.5,最適反應溫度50℃;米氏常數22.63mg/L,與游離(34.62mg/L)相比,對底物的親和力增強;室溫時操作半衰期較游離酶延長6d以上;經過8次重複操作,酶活損失低於5%。由此可見,固定化酶提高了游離酶的貯藏和操作穩定性。

English Abstract

After being dissolved in the high concentration of NaOH, a-chitin could be prepared into α-chitosan of three different kinds of degree of deacetylation. Glucoamylase could be coupled into the carriers when the above-mentioned materials were grafted with glutaraldehyde, thus the immobilizations of glucoamylase were available. The research on the properties of the immobilized enzymes and dynamical constant indicated, the capability of the immobilized enzymes adopting α-chitosan with the highest degree of deacetylation as the carrier was best, whose enzyme recovery was 75%. The optimal pH value of the immobilized glucoamylase was 6.0~7.5, higher than that of the free enzyme. The optimal temperature was 50℃. The Michaelis constant of the immobilized glucoamylase was 22.63mg/L. Compared to the free enzyme (34.62mg/L), the appetency of the immobilized enzymes to the substrate increased. The operation half-life of the immobilized enzymes in room temperature delayed more than 6d to the free enzyme. The enzyme activity of the immobilized enzymes lost less than 5% after 8 times' repeated operation. Thus it could be seen that the immobilized glucoamylase improved the store stability and operation stability of the free enzyme.

Topic Category 工程學 > 化學工業