豬肉、雞肉及其內臟中磺胺劑殘留量分析方法之探討

An Investigation of Sulfa-Drugs Residues in Swine and Chicken Organs

蔡佳芬(Chia-Fen Tsai) ； 鄭秋真(Chieu-Chen Cheng) ； 周薰修(Shin-Shou Chou)

《藥物食品檢驗局調查研究年報》 9號 (1991/09) Pp. 312-320

https://doi.org/10.6946/ASRNL.199109.0312

摘要

行政院衛生署已於76年4月公告『動物用藥殘留標準』，急須建立禽言產品中殘留藥物檢驗方法，並了解國內畜產品中藥物殘留現況。經分別比較了薄層層析法，Densitometer定量法，美國農部之肉品中殘留磺胺劑試驗法及高效液相層析法，結果顯示薄層層析法可明顯地分離磺胺噻啶、磺胺二甲嘧唑、磺胺一甲氧嘧啶、磺胺二甲氧嘧啶、磺胺奎林等五種磺胺劑，但若以Densitometer來加以定量，則因時間的長短會影響其螢光強度，而導致偵測誤差。而美國農部所採用的試驗法於回收試驗時顯示，磺胺噻唑之回收率僅約20%，顯然偏低。利用高效液相層析法時將五種磺胺劑標準品添加於樣品中使其最終濃度為0.05~0.30ppm後，其平均回收率分別為豬肉中93.5~114.2%；豬肝中86.3~96.7%；豬腎中94.5~114.2%；雞肉中96.0~102.5%；雞肝中50.9~130.4%。回收情形最為理想。因而建立了以薄層層析法為定性方法而以高效液相層析法為定量方法，達快速篩檢之功效。五種磺胺劑以薄層層析法為定性方法時，其最低檢出量為0.005ppm，以高效液相層析法為定量方法時，其最低檢出量為0.01ppm。

並列摘要

Comparison of thin layer chromatography (TLC), FSIS-USDA method and high performance liquid chromatography (HPLC), in the determination of sulfa-drugs in animal tissues were first made. Then a method of simuitaneous determination was established for a qualitative TLC and a quantitative HPLC assay of residues of sulfathiazole, sulfamethazine, sulfamonomethoxine, sulfadimethoxine and sulfaquinoxaline in swine and chicken organs. Average recoveries of sulfathiazole, sulfamethazine, sulfamonornethoxine, sulfadimethoxine and sulfaquinoxaline added to swine muscle, swine liver, swine kidney, chicken muscle and chicken liver were 93.5-114.2%, 86.3-96.7%, 94.5-114.2%, 96.0-102.5% and 80. 9-130.4%, respectively at the 0.05.0.3ug/g level. Determination limits of thin layer chromatographic method and high performance liquid chromatographic method were 0.005 and 0.0lug/g, respectively.