- 期刊
Enhancement of Glucose Uptake in 3T3-Ll Adipocytes by Toona Sinensis Leaf Extract
香椿葉抽出物增加3T3-L1脂肪細胞對葡萄糖攝取之研究
摘要
本實驗主要題探討使用50%酒精溶液抽取香椿葉的萃取物對3T3-L1脂肪細胞對[3H]-2-deoxyglucose攝取量的影響。當細胞經過不同濃度的香椿葉萃取物(0.001、0.01及0.1mg/mL)經過60分鐘處理後，脂肪細胞對葡萄糖的攝取量由控制組的0.23nmol/min/mg protein增加到0.30、0.33及0.38的攝取量，然而在胰島素存在下，細胞對葡萄糖的攝取量會增加到0.35，而香椿葉萃取物會更進一步使細胞對葡萄糖攝取量增加到0.41、0.46及0.52nmol/min/mg protein。若3T3-L1脂肪細胞預先經過20mM葡萄糖處理48小時後，雖然細胞對葡萄糖攝取量降低，但是香椿葉萃取物也會增加細胞對葡萄糖的攝取。在胰島素存在下cycloheximide 及 calphostin C不會制香椿葉萃取物增加細胞葡萄糖攝取的活性。由以上結果顯示，有胰島素或沒有胰島素的培養液中，香椿葉萃取物都會增加3T3-L1脂肪細胞對葡萄糖的攝取量，但是兩者可能透過不同調節機轉。
並列摘要
The effects of substances extracted from Toona sinensis leaves, using 50% alcohol / water, on cellular [3H]-2-deoxyglucose uptake in differentiated cultured 3T3-Ll adipocytes were Investigated. Following treatment of cells with 0.001, 0.01, or 0.1 mg / mL extracts for 60 minutes, [3H]-2-deoxyglucose uptake increased from a basal value of 0.23 nmol / mini mg protein to 0.30, 0.33, and 0.38 nmol /min / mg protein, respectively. In insulin-stimulated cells, cellular [3H]-2-deoxyglucose uptake was enhanced by Toona sinensis leaf extract from a basal value of 0.35 nmol / min / mg protein to 0.41, 0.46, and 0.52 nmol / min / mg protein, respectively. Cellular glucose uptake was also enhanced by Toona sinensis leaf extract after incubation of cells with 20 mM glucose for 48 hours. Cellular glucose uptake with a combination of Toona sinensis leaf extract and insulin was significantly inhibited by pretreatment of cells with the protein synthesis inhibitor cycloheximide and the protein kinase C inhibitor calphostin C in normal-, medium- and high-glucose media. However, the glucose uptake-enhancing effect of Toona sinensis leaf extract was not diminished by cycloheximide and calphostin C in the absence of insulin. These results indicate that enhancement of cellular glucose uptake by Toona sinensis leaf extract in basal and insulinstimulated 3T3-Ll adipocytes may be mediated by distinct mechanisms.
延伸閱讀
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