Title

东海微生物中6种环二肽类天然活性物质的分离和鉴定

Translated Titles

Bioactive Cyclodipeptides Extracted from Marine Microbes in East China Sea

Authors

艾峰(Feng Ai);许强芝(Qiang-Zhi Xu);杨妤(Yu Yang);刘小宇(Xiao-Yu Liu);施晓琼(Xiao-Qiong Shi);宋志刚(Zhi-Gang Song);焦炳华(Bing-Hua Jiao)

Key Words

环二肽 ; 海洋生物学 ; 稻瘟霉 ; 鳗弧菌 ; cyclodipeptide ; marine biology ; Pyricularia Oryzae ; Vibrio anguillarm

PublicationName

第二軍醫大學學報

Volume or Term/Year and Month of Publication

27卷1期(2006 / 01 / 20)

Page #

22 - 24

Content Language

簡體中文

Chinese Abstract

目的:从海洋微生物中寻找天然活性物质。方法:利用稻瘟霉作为菌株筛选模型,对筛选出来的活性菌株F8712发酵液的醋酸乙酯提取物进行化合物分离;通过1HNMR、13CNMR和ESI-MS对化合物进行结构鉴定。结果和讨论:鉴定了6个环二肽类化合物,它们分别为环[Ala-Leu](Ⅰ),环[Ala-Ile](Ⅱ),环[Val-Pro](Ⅲ),环[leu-Pro](Ⅳ),环[Ile-Pro](Ⅴ)和环[Leu-Val](Ⅵ)。这些环二肽都没有抗稻瘟霉活性,化合物Ⅴ对鳗弧菌(Vibrio anguillarm)具有较强的抑制能力,其MIC为0.07μg/ml。

English Abstract

Objective: To search for bioactive compounds from marine microbes. Methods Pyricularia oryzae was used to screen microbes. Compounds extracted with EtOAc from the fermentation broth of F8712 strain (an active microbe screened out) were separated. The chemical structures of the separated compounds were identified by 1HNMR, 13CNMR and ESI-MS techniques. Results: Six cyclic dipeptides, cyclo (Ala-Leu), cyclo(Ala-Ile), cyclo(Ile-Pro), cyclo(Val-Pro), cyclo(Leu-Pro) and cyclo(Leu-Val), were obtained. Conclusion: None of the 6 cyclodipeptides has any anti-Pyriculatia oryzae activities. Compound Ⅴ has potent inhibitory effects on Vibrio anguillarm with a minimum inhibitory concentration of 0.07μg/ml.

Topic Category 醫藥衛生 > 醫藥衛生綜合
醫藥衛生 > 預防保健與衛生學
醫藥衛生 > 中醫藥學
醫藥衛生 > 藥理醫學
醫藥衛生 > 社會醫學