Title

高锌对Caco-2细胞铁、锌含量及其调控基因mRNA表达的影响

Translated Titles

Effect of High Zinc Concentrations on Contents of Iron and Zinc and Expression of Their Regulating mRNA in Caco-2 Cells

Authors

张弌(Yi Zhang);李正银(Zheng-Yin Li);Jing Li;秦海宏(Hai-Hong Qin)

Key Words

锌 ; 铁 ; Caco-2细胞 ; DMT1 ; IREG1 ; ZnT1 ; hZIP4 ; 基因表达 ; zinc ; iron ; Caco-2 cells ; DMT1 ; IRT1 ; ZnT1 ; hZIP4 ; gene expression

PublicationName

第二軍醫大學學報

Volume or Term/Year and Month of Publication

27卷1期(2006 / 01 / 20)

Page #

41 - 45

Content Language

簡體中文

Chinese Abstract

目的:探讨高锌对小肠细胞铁、锌含量和铁、锌调控基因DMT1、IREG1、ZnT1及hZIP4 mRNA表达的影响。方法:以Caco-2细胞为小肠细胞体外模型,以锌浓度分别为注4、50、l00、200μmol/L的培养液培养别24h,用原子吸收分光光度计检测细胞内铁、锌含量,用RT-PCR方法检测DMT1、IREG1、ZnT1及hZiP4 mRNA表达,以GAPDH mRNA水平作为内参照。结果:高锌处理24h后Caco-2细胞内锌含量升高,培养基锌浓度为50μmol/L时达到高峰,而细胞内铁含量却随培养基锌浓度升高而降低。细胞内DMT1、IREG1、ZnT1 mRNA表达均出现上调,hZIP4 mRNA表达则发生下凋。结论:补锌可以提高小肠细胞内锌含量,但会使细胞内铁含量下降。铁和锌在小肠中的吸收可能相互影响。

English Abstract

Objective To observe the effect of high zinc concentrations on contents of iron zinc and expression of their regulating mRNA in Caco-2 cells. Methods: Caco-2 cells were used as model of human small intestina1 enterocytes and were treated for 24h with 4, 50, 100 and 200μmol/L of zinc. The zinc and iron contents were determined by atom absorption spectrophotography and the expression of DMT1, IREG1, ZnT1 and hZIP4 mRNA were determined by RT-PCR(the level of GAPDH was taken as internal control). Results: The zinc content in Caco-2 cells was increased 24h after treatment with zinc, and reached the peak when exposed to 5oμmol/L zinc. The iron content decreased with the increase of zinc concentration. Zinc supplement increased the expressions of DMT1, IREG1 and ZnT1 mRNA but decreased the expression of hZIP4 mRNA. Conclusion: It is suggested that zinc supplement can increase the contents of zinc but decrease the contents of iron in Caco-2 cells. There may be interactions between iron and zinc in the intestinal lumen.

Topic Category 醫藥衛生 > 醫藥衛生綜合
醫藥衛生 > 預防保健與衛生學
醫藥衛生 > 中醫藥學
醫藥衛生 > 藥理醫學
醫藥衛生 > 社會醫學