Identification of Androgen-Responsive Element (ARE) and Sp1 Element in the Maspin Promoter

Mei-Lan He ； An-Li Jiang ； Peng-Ju Zhang ； Xiao-Yan Hu ； Zhi-Fang Liu ； Hui-Qing Yuan ； Jian-Ye Zhang

《The Chinese Journal of Physiology》 48卷3期 (2005/09) Pp. 160-167

並列摘要

Maspin is a senile protease inhibitor (serpin) with tumor-suppressing function in mammary gland. It is down-regulated in primary prostate cancer cells and lost in metastatic cells. To better understand the transcriptional regulation of maspin gene, the 860bp (-765~+95) of its promoter sequence was amplified b PCR from the human genomic DNA. Then this 860bp sequence and a series of deletions from 5' and 3' ends were inserted into the upstream of luciferase reporter gene respectively. Results from dual luciferase reporter assay and electrophoretic mobility shift assay indicated that there were a negative androgen-responsive element (ARE) in the region of -277 to -262 and a positive Sp1 element in the region of +14 to +35, respectively. In addition, androgen receptor (AR) can recognize and bind to the ARE element, and then inhibit the activity of maspin promoter.

並列關鍵字

maspin promoter ； prostate cancer ； androgen responsive element ； Sp1 element

參考文獻

Alan, H.,David, J.A.,Christopher, D.,Xu, S.W.,Carol, M.B.,Andrew, L.(2003).Constitutive connective tissue growth factor expression in scleroderma fibroblasts is dependent on Spl.J. Biot. Chem.278,41728-41733.

Domann, F.E.,Rice, J.C.,Hendrix, M.J.,Futscher, B.W.(2000).Epigenetic silencing of maspin gene expression in human breast cancers.Int. J. Cancer.85,805-810.

Hagen, G.,Muller, S.,Beato, M.,Suske, G.(1992).Cloning by recognition site screening of two novel GT box binding proteins: a family of Spi related genes.Nucl. Acids. Res..20,5519-5525.

Hour, T.C.,Chen, J.,Huang, C.Y.,Guan, J.Y.,Lu, S.H.,Pu, S.H.(2002).Curcumin enhances cytotoxicity of chemotherapeutic agents in prostate cancer cells by inducing p2l(superscript WAFI/CIPI) and C/EBPβ expression and suppressing NF(subscript -K)B activation.Prostate.51,211-218.

Jiang, N.,Meng, Y.,Zhang, S.,Mensah, O.E.,Sheng, S.(2002).Maspin sensitizes breast carcinoma cells to induced apoptosis.Oncogene.26,4089-4098.