Title

The Antihypertensive Activity of Angiotensin-Converting Enzyme Inhibitory Peptide Containing in Bovine Lactoferrin

Authors

Nai-Yuan Lee;Juei-Tang Cheng;Toshiki Enomoto;Ichiro Nakamura

Key Words

angiotensin-converting enzyme ACE inhibitory peptide ; bovine lactoferrin ; captopril ; spontaneously hypertensive rat

PublicationName

The Chinese Journal of Physiology

Volume or Term/Year and Month of Publication

49卷2期(2006 / 04 / 01)

Page #

67 - 73

Content Language

英文

English Abstract

Angiotensin I-converting enzyme (ACE) inhibitory peptide was isolated from the bovine lactoferrin hydrolysate using peptic hydrolysis by 2-step of reverse-phase high-performance liquid chromatography. This peptide was identified as Leu-Arg-Pro-Val-Ala-Ala and it produced a concentration-dependent inhibition of ACE activity in vitro with an IC50 value of about 4.14 μM. Also, this inhibition was identified as noncompetitive from the Lineweaver-Burk plot. Moreover, the antihypertensive activity of Leu-Arg-Pro-Val-Ala-Ala was investigated by the intravenous injection into spontaneously hypertensive rats (SHRs). A dose-dependent reduction of systolic blood pressure by this peptide was observed at 60 min after injection and it maximally decreased the blood pressure at a rate of 1 nmol/ml/kg. The blood pressure lowering activity of this peptide was calculated as 210% of captopril (10 pmol/ml/kg) that was used as positive control. Otherwise, identification of this peptide in the blood of SHRs was carried out chromatographically. Reduction of blood pressure coincides with the peak peptide concentration in the serum. Thus, we conclude that this peptide inhibits ACE activity in vitro and lowers systolic blood pressure in spontaneously hypertensive rat.

Topic Category 醫藥衛生 > 基礎醫學
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