Effects of Benzo (a) Pyrene and Ethanol on Oxidative Stress of Brain, Lung Tissues and Lung Morphology in Rats

Memet Hanifi Emre ； Göknur Aktay ； Aladdin Polat ； Nigar Vardι

《The Chinese Journal of Physiology》 50卷3期 (2007/06) Pp. 143-148

並列摘要

Ethanol and benzo(a)pyrene cause lipid peroxidation either by producing the reactive oxygen species or decreasing the activities of antioxidant enzymes that lead to cellular damage and cellular dysfunction. In this study, we investigated both physiological and histological changes in lung and physiological changes in brain after the administration of benzo(a)pyrene and ethanol both separately and together. Male Sprague Dawley rats were divided into four groups, each containing seven rats as follows: Group Ⅰ (control), group Ⅱ (benzo(a)pyrene, [B(a)P]), group Ⅲ ([B(a)P] + ethanol (EtOH)) and group Ⅳ (EtOH). Superoxide dismutase (SOD) activity, levels of glutathione (GSH), malondialdehyde (MDA) as well as histological examinations were evaluated to demonstrate the damages in lung and brain tissues following the administration of [B(a)P] and EtOH. SOD activities of lung and brain tissues in group Ⅱ and group Ⅲ decreased significantly, compared to that in group Ⅰ and group Ⅳ, respectively. GSH levels of both the lung and brain tissues in the experimental groups were lower when compared to the control group. MDA levels of lung tissues in group Ⅱ and Ⅲ were significantly higher than that in the control group. Moreover, MDA levels in the brain tissues of all the experimental groups were higher than that in the control group, but these values were only significantly higher in group Ⅱ and Ⅳ. In the second study group, [B(a)P] administration resulted in lung damage. On the other hand, lung tissue of the third experimental group showed moderate damage, and lung tissues of the fourth group was less severely damaged. [B(a)P] and EtOH administration alone or together caused changes in the GSH, MDA levels and SOD enzyme activity in the lung and brain tissues. We also noted that [B(a)P] and EtOH caused different degrees of histological changes.

並列關鍵字

brain ； lung, ethanol ； benzo(a)pyrene ； SOD ； MDA

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