Anti-Inflammatory Effects of LK-3, on LPS-Induced Sepsis in Rats


Wen-Hui Tsai;Pao-Yun Cheng;Yen-Mei Lee;Min-Chen Chiu;Shyi-Shiaw Jiau;Edwin S.C. Wu;Mao-Hsiung Yen

Key Words

dextromethorphan ; reactive oxygen species ; sepsis ; TNF-α ; nitric oxide ; circulatory failure


The Chinese Journal of Physiology

Volume or Term/Year and Month of Publication

51卷5期(2008 / 10 / 01)

Page #

292 - 300

Content Language


English Abstract

Dextromethorphan (DM), an antitussive agent, has been shown to have anti-inflammatory and immunomodulatory effects in vitro. Thus, the aim of this study was to evaluate the effects of LK-3, an analog of DM, on sepsis induced by intravenous (i.v.) administration of lipopolysaccharide (LPS; 10 mg/kg) in anesthetized Wistar rats. Results demonstrated that post-treatment with LK-3 (4 mg/kg, i.v.) significantly attenuated the deleterious hemodynamic changes (e.g., hypotension and bradycardia) in rats treated with LPS. Meanwhile, LK-3 (4 mg/kg) significantly inhibited the elevation of plasma tumor necrosis factor-α, as well as values of glutamate-oxalacetate transaminase (GOT) and glutamatepyruvate transaminase (GPT) caused by LPS. The induction of inducible NO synthase and the overproduction of NO and superoxide anions by LPS were also reduced by post-treatment of LK-3. Moreover, infiltration of neutrophils into the lungs and liver of rats 8 h after treatment with LPS was also reduced by post-treatment with LK-3. In conclusion, the beneficial effects of LK-3 on LPS-induced sepsis resulted from its anti-inflammatory and antioxidant effects.

Topic Category 醫藥衛生 > 基礎醫學
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