Supplementary Catechins Attenuate Cooking-Oil-Fumes-Induced Oxidative Stress in Rat Lung




Chao-Huei Yang;Chun-Yao Lin;Joan-Hwa Yang;Shaw-Yih Liou;Ping-Chia Li;Chiang-Ting Chien

Key Words

catechins ; reactive oxygen species ; HSP70 ; cooking-oil-fumes ; apoptosis ; lung


The Chinese Journal of Physiology

Volume or Term/Year and Month of Publication

52卷3期(2009 / 06 / 01)

Page #

151 - 159

Content Language


English Abstract

Cooking-oil-fumes containing toxic components may induce reactive oxygen species (ROS) to oxidize macromolecules and lead to acute lung injury. Our previous study showed that a decaffineated green tea extract containing (+)-catechin, (-)-epicatechin, (+)-gallocatechin, (-)-epigallocatechin, (-)-epicatechin gallate, and (-)-epigallocatechin gallate can inhibit oxidation, inflammation, and apoptosis. We determined whether the catechins supplement may reduce cooking-oil-fumes-induced acute lung injury in rat. In the urethane-anesthetized Wistar rat subjected to 30-120 min of cooking-oil-fumes exposure, blood ROS significantly increased in the recovery stage. After 30-min cooking-oil-fumes exposure, the enhanced blood ROS level further increased in a time-dependent manner during the recovery stage (321±69 counts/10 s after 1 h, 540±89 counts/10 s after 2 h, and 873±112 counts/10 s after 4 h). Four hours after 30-min cooking-oil-fumes exposure, lung lavage neutrophils and ROS as well as lung tissue dityrosine and 4-hydroxy-2-nonenal increased significantly. Two weeks of catechins supplememnt significantly reduced the enhanced lavage ROS, lung dityrosine and 4-hydroxy-2-nonenal level. Cooking-oil-fumes-induced oxidative stress decreased lung Bcl-2/Bax ratio and HSP70 expression, but catechins treatment preserved the downregulation of Bcl-2/Bax ratio and HSP70 expression. We conclude that catechins supplement attenuates cooking-oil-fumes-induced acute lung injury via the preservation of oil-smoke induced downregulation of antioxidant, antiapoptosis, and chaperone protein expression.

Topic Category 醫藥衛生 > 基礎醫學
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Times Cited
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