Aging Effects on the BDNF mRNA and TrkB mRNA Expression of the Hippocampus in Different Durations of Stress

Shu-Hong Shao ； Shou-Sen Shi ； Zun-Ling Li ； Ming-Shan Zhao ； Shu-Yang Xie ； Fang Pan

《The Chinese Journal of Physiology》 53卷5期 (2010/10) Pp. 285-293

https://doi.org/10.4077/CJP.2010.AMK056

並列摘要

Brain-derived neurotrophins factor (BDNF) belongs to the neurotrophins family which acts on neuronal survival and growth and has been associated with cognition process. TrkB is the primary signal transduction receptor for BDNF. In the present study, hippocampal BDNF and TrkB mRNA were detected by RT-PCR in 2-and 22-month rats, respectively, which were exposed to different durations of mild stress protocol of 8-day, 21-day and 28-day chronic unpredictable mild stress (CUMS). Observation of exploratory behavior in an open field (OF) test indicated stress levels and changes of spontaneous activity. We demonstrated that CUMS induced decrease of BDNF mRNA in two aged groups, but the increase change of TrkB mRNA compared with those of the control groups. Moreover, the changes of BDNF mRNA and TrkB mRNA measured in both the 21-day and 28-day stress groups represent obvious decrease than those of the 8-day stress groups, and the expression examined in young groups appeared to be higher than those of the aged group, especially in the 28-day stress groups. Results of OF test showed that explicit behaviors in two age groups decreased gradually with the process of stress revealing a depressive state under the stress condition. Meanwhile, the behaviors of young rats seemed to be more active than those of the aged rats, exhibiting weak adaptation to the stress. The study suggested that stress paradigm and aging certainly had effect on the regulation of BDNF mRNA and TrkB mRNA which might be related to damage and protection function of the hippocampus.

並列關鍵字

BNDF ； hippocampus ； behavior ； senescence

參考文獻

Adlard, P. A.,Cotman, C. W.(2004).Voluntary exercise protects against stress-induced decreases in brain- derived neurotrophic factor protein expression.Neuroscience.124,985-992.

Aggleton, J. P.,Vann, S. D.,Oswald, C. J.,Good, M.(2000).Identifying cortical inputs to the rat hippocampus that subserve allocentric spatial processes: a simple problem with a complex answer.Hippocampus.10,466-474.

Alberts, S. C.,Sapolsky, R. M.,Altmann, J.(1992).Behavioral, endocrine, and immunological correlates of immigration by an aggressive male into a natural primate group.Horm. Behav..26,167-178.

Altar, C. A.(1999).Neurotrophins and depression.Trends Pharmacol. Sci..20,59-61.

Baker, S. L.,Kentner, A. C.,Konkle, A. T.,Santa-Maria Barbagallo, L.,Bielajew, C.(2006).Behavioral and physiological effects of chronic mild stress in female rats.Physiol. Behav..87,314-322.