Aging Effects on the BDNF mRNA and TrkB mRNA Expression of the Hippocampus in Different Durations of Stress


Shu-Hong Shao;Shou-Sen Shi;Zun-Ling Li;Ming-Shan Zhao;Shu-Yang Xie;Fang Pan

Key Words

BNDF ; hippocampus ; behavior ; senescence


The Chinese Journal of Physiology

Volume or Term/Year and Month of Publication

53卷5期(2010 / 10 / 31)

Page #

285 - 293

Content Language


English Abstract

Brain-derived neurotrophins factor (BDNF) belongs to the neurotrophins family which acts on neuronal survival and growth and has been associated with cognition process. TrkB is the primary signal transduction receptor for BDNF. In the present study, hippocampal BDNF and TrkB mRNA were detected by RT-PCR in 2-and 22-month rats, respectively, which were exposed to different durations of mild stress protocol of 8-day, 21-day and 28-day chronic unpredictable mild stress (CUMS). Observation of exploratory behavior in an open field (OF) test indicated stress levels and changes of spontaneous activity. We demonstrated that CUMS induced decrease of BDNF mRNA in two aged groups, but the increase change of TrkB mRNA compared with those of the control groups. Moreover, the changes of BDNF mRNA and TrkB mRNA measured in both the 21-day and 28-day stress groups represent obvious decrease than those of the 8-day stress groups, and the expression examined in young groups appeared to be higher than those of the aged group, especially in the 28-day stress groups. Results of OF test showed that explicit behaviors in two age groups decreased gradually with the process of stress revealing a depressive state under the stress condition. Meanwhile, the behaviors of young rats seemed to be more active than those of the aged rats, exhibiting weak adaptation to the stress. The study suggested that stress paradigm and aging certainly had effect on the regulation of BDNF mRNA and TrkB mRNA which might be related to damage and protection function of the hippocampus.

Topic Category 醫藥衛生 > 基礎醫學
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