Antioxidant Effects of Melatonin and Coenzyme Q10 on Oxidative Damage Caused by Single-Dose Ochratoxin A in Rat Kidney

Aydin Yenilmez ； Burhanettin Isikli ； Erinc Aral ； Irfan Degirmenci ； Emine Sutken ； Cengiz Baycu

《The Chinese Journal of Physiology》 53卷5期 (2010/10) Pp. 310-317

https://doi.org/10.4077/CJP.2010.AMK073

並列摘要

In the study, the effects of relatively high single-dose of Ochratoxin A (OTA) and the antioxidant effects of Melatonin (Mel) and Coenzyme Q10 (CoQ10) on OTA-induced oxidative damages in rats were investigated. A total of 28 male Sprague-Dawley rats were divided into four groups of 7 rats each: Control, OTA, Mel+OTA and CoQ10+OTA groups. Malondialdehyde (MDA) levels in the plasma and glutathione (GSH) levels in whole blood were measured; kidneys (for histological inspection and for apoptosis detection by TUNEL method) and bone marrow samples (for chromosome aberration and mitotic index) were taken. The rats in the OTA group showed limited degeneration of tubular cells. In some tubules karyomegaly, desquamated cells and vacuolization were observed by light microscopy. Mel and CoQ10 treatment significantly reduced the severity of the lesions. MDA levels of the OTA group were significantly higher than the control, OTA+Mel and OTA+CoQ10 groups, while GSH levels were significantly lower than the control, OTA+Mel and OTA+CoQ10 groups. Higher incidences of apoptotic bodies were observed in the kidneys of the OTA group although OTA administration did not significantly change the incidence of apoptotic bodies when compared to the control and antioxidant administrated groups. Although the percentage of the mitotic index was lowest in the OTA group, no statistical difference was found among the groups. Additionally, OTA had no numerical and structural significant effects on chromosomes. It was observed that single-dose OTA administration caused oxidative damages in rat kidney and Mel or CoQ10 treatment appeared to ameliorate the OTA-induced tissue injuries.

並列關鍵字

coenzyme Q10 ； melatonin ； mitotic index ； ochratoxin A ； oxidative stress

參考文獻

Abdel-Wahhab, M. A.,Abdel-Galil, M. M.,El-Lithey, M.(2005).Melatonin counteracts oxidative stress in rats fed an ochratoxin A contaminated diet.J. Pineal Res..38,130-135.

Albassam, M. A.,Yong, S. I.,Bhatnagar, R.,Sharma, A. K.,Prior, M. G.(1987).Histopathologic and electron microscopic studies on the acute toxicity of ochratoxin A in rats.Vet. Pathol..24,427-435.

Atroshi, F.,Biese, I.,Saloniemi, H.,Ali-Vehmas, T.,Saari, S.,Rizzo, A.,Veijalainen, P.(2000).Significance of apoptosis and its relationship to antioxidants after ochratoxin A administration in mice.J. Pharm. Pharm. Sci..3,281-291.

Aydin, G.,Ozcelik, N.,Cicek, E.,Soyoz, M.(2003).Histopathologic changes in liver and renal tissues induced by Ochratoxin A and melatonin in rats.Hum. Exp. Toxicol..22,383-391.

Beutler, E.(1957).The glutathione instability of drug-sensitive red cells: a new method for the in vitro detection of drug sensitivity.J. Lab. Clin. Med..49,84-95.