Herbal Supplement Attenuation of Cardiac Fibrosis in Rats with CCl4-Induced Liver Cirrhosis

Hsiao-Chuan Chang ； Yung-Wei Chiu ； Yueh-Min Lin ； Ray-Jade Chen ； James A. Lin ； Fuu-Jen Tsai ； Chang-Hai Tsai ； Yu-Chun Kuo ； Jer-Yuh Liu ； Chih-Yang Huang

《The Chinese Journal of Physiology》 57卷1期 (2014/02) Pp. 41-47

https://doi.org/10.4077/CJP.2014.BAB147

並列摘要

Previously we found carbon tetrachloride (CCl4) induced cirrhosis associated cardiac hypertrophy and apoptosis. The purpose of this study is to determine whether further CCl4 treatment would induce cardiac cell fibrosis. The cardiac tissues were analyzed by H&E. histological staining, Trichrome Masson staining and Western blotting. The results showed that the CCl4-treated-only group exhibits more trichrome staining, meaning that more fibrosis is present. Moreover, CCl4 could further induce cardiac-fibrosis via TGF-β-p-Smad2/3-CTGF pathway. However, our data showed that the CCl4- indcued cardiac abnormalities were attenuated by Ocimum gratissimum extract (OGE) and silymarin cotreatments. In conclusion, our results indicated that the OGE and silymarin may be a potential traditional herb for the protection of cardiac tissues from the CCl4 induced cirrhosis associated cardiac fibrosis through modulating the TGF-β signaling pathway.

並列關鍵字

cardiac fibrosis ； CCl4 ； Ocimum gratissimum ； silymarin ； TGF-β

參考文獻

Abreu, J.G.,Ketpura, N.I.,Reversade, B.D.,Robertis, E.M.(2002).Connective-tissue growth factor (CTGF) modulates cell signaling by BMP and TGF-β.Nat. Cell Biol..4,599-604.

Aguiyi, J.C.,Obi, C.I.,Gang, S.S.,Igweh, A.C.(2000).Hypoglycaemic activity of Ocimum gratissimum in rats.Fitoterapia..71,444-446.

Atal, C.K.,Sharma, M.L.,Kaul, A.,Khajuria, A.(1986).Immunomodulating agents of plant origin. I: Preliminary screening.J. Ethnopharmacol..18,133-141.

Au, C.G.,Butler, T.L.,Sherwood, M.C.,Egan, J.R.,North, K.N.,Winlaw, D.S.(2011).Increased connective tissue growth factor associated with cardiac fibrosis in the mdx mouse model of dystrophic cardiomyopathy.Int. J. Exp. Pathol..92,57-65.

Ayisi, N.K.,Nyadedzor, C.(2003).Comparative in vitro effects of AZT and extracts of Ocimum gratissimum, Ficus polita, Clausena anisata, Alchornea cordifolia, and Elaeophorbia drupifera against HIV-1 and HIV-2 infections.Antiviral Res..58,25-33.