Title

大荳葉片衰老過程中半胱氨酸蛋白酶基因的特異性表達

Translated Titles

Specific Expression of Cysteine Protease Genes during Soybean Leaf Senescence

Authors

王勇(Yong Wang);王寧寧(Ning-Ning Wang);王淑芳(Shu-Fang Wang);張韌(Ren Zhang);朱亮基(Liang-Ji Zhu)

Key Words

大荳 ; 葉片衰老 ; 特異表達基因 ; 半胱氨酸蛋白酶基因 ; soybean ; leaf senescence ; specific expressed gene ; cysteine protease gene

PublicationName

南開大學學報(自然科學版)

Volume or Term/Year and Month of Publication

30卷3期(1997 / 09 / 01)

Page #

106 - 108

Content Language

簡體中文

Chinese Abstract

用反轉錄和多聚酶鏈式反應(RT-PCR)和瓊脂糖凝膠電泳方法對大荳葉片衰老過程中半胱氨酸蛋白酶基因的表達情況進行了研究,發現由PCR擴增出的cDNA帶譜會隨著葉片的不斷衰老而發生較明顯的改變。初步證明,至少有一條cDNA帶為綠葉樣品所特有,另外兩條cDNA帶則為衰老葉片樣品所特有,該實驗結果為進一步分離大荳葉片衰老時特異表達基因及其啟動子和最終實施轉基因工程奠定了基礎。

English Abstract

Using RT-PCR and agarose gel electrophoresis methods, the expression of cysteine protease genes during soybean leaf senescence was investigated. Differential cDNA bands were found on the gel between green leaf sample and senescence leaf sample. Preliminary work showed that one cDNA band was green leaf specific and two others, on the other hand, senescence specific. These results are very important for isolating senescence specific gene promoters and further gene transfer work.

Topic Category 基礎與應用科學 > 基礎與應用科學綜合
基礎與應用科學 > 數學
基礎與應用科學 > 資訊科學
基礎與應用科學 > 物理
基礎與應用科學 > 化學
醫藥衛生 > 醫藥衛生綜合
生物農學 > 生物農學綜合