菜豆普通嵌紋病毒（BCMV）與胡瓜嵌紋病毒（CMV）的萊豆（Phaseolus limensis）分離株之鑑定與發生

Occurrence and Identification of Bean Common Mosaic Virus and Cucumber Mosaic Virus Isolated from Lima Bean (Phaseolus limensis)

鄧汀欽(T. C. Deng) ； 蔡錦慧(C. H. Tsai) ； 蔡惠玲(H. L. Tsai) ； 黃文慶(W. C. Huang) ； 宋妤(Y. Sung)

《植物保護學會會刊》 55卷4期 (2013/12) Pp. 105-116

https://doi.org/10.6715/PPB.201312_55(4).0001

並列摘要

Two virus isolates, BCMV-c9 and CMV-Lima, were isolated from diseased lima bean plants (Phaseolus limensis Macf.) cultivated in Taichung and Tainan. After local-lesion isolation on Chenopodium quinoa, BCMV-c9 was identified as a Bean common mosaic virus (BCMV) by ELISA and CMV-Lima was a Cucumber mosaic virus (CMV) tested by SDS-immunodiffusion. For further identification, potyvirus specific primer (Pot1/HRP5) and cucumovirus specific primer (CPTALL-3 / CPTALL-5) were adopted for reverse transcription polymerase chain reaction (RT-PCR). By nucleotide sequence alignment, the CP gene of BCMV-c9 was 89.2% identical to that of Accession number U34972, a BCMV-PSt strain. Phylogenetic analysis also showed that BCMV-c9 was closer to BCMV-PSt strains than to BCMV-BlCM strains. CMV-Lima shared more than 95% of identities with CMV isolates Tfn and NT9 which belong to IB subgroup, 93-94% with isolates Fny and Y which belong to IA subgroup, and only 77-78% with isolates Q and LS which belong to IA subgroup. Consequently, BCMV-c9 is identified as a BCMV-PSt strain and CMV-Lima is a CMV strain that belongs to subgroup IB. From the field survey showed that BCMV was widespread in lima bean cultivated areas, and CMV was occasionally occurred.

並列關鍵字

BCMV-BlCM ； BCMV-PSt ； strain ； subgroup ； phylogenetic analysis ； Taiwan