Title

大豆耐鹽基因的PCR標記

Translated Titles

Tagging Salt Tolerant Gene Using PCR Markers in Soybean

Authors

郭蓓(Bei Guo);邱麗娟(Li-Juan Qiu);邵桂花(Gui-Hua Shao);常汝鎮(Ru-Zhen Chang);劉立宏(Li-Hong Liu);許占友(Zhan-You Xu);李向華(Xiang-Hua Li);孫建英(Jian-Ying Sun)

Key Words

耐鹽基因 ; 分子標記 ; 專利 ; 大豆 ; Salt tolerant gene ; Molecular marker ; Patent ; Soybean

PublicationName

中國農業科學

Volume or Term/Year and Month of Publication

33卷1期(2000 / 02 / 01)

Page #

10 - 16

Content Language

簡體中文

Chinese Abstract

以大豆耐鹽品種和鹽敏感品種及“耐鹽品種×鹽敏感品種”組合的F2群體爲試驗材料,篩選和鑒定與大豆耐鹽性基因緊密連鎖的PCR標記。旨在建立快速准確的大豆耐鹽性鑒定方法。利用BSA法,對耐(敏)鹽品種池和一個組合F2的耐(敏)鹽池進行了鑒定。獲得一個共顯性標記。經F2分析,在鹽敏感個體中僅擴增出約600bp的特異片段;在耐鹽性個體中擴增出約700bp的特異片段或2個特異片段(700bp/600bp),經邊連鎖值測定,表明該標記與大豆耐鹽基因位點緊密連鎖。此外,該標記在其它2個組合F2群體及12個耐鹽品種和13個鹽敏感品種中得到驗證,表明此標記可用於大豆耐鹽種質鑒定及大豆耐鹽遺傳育種的分子標記輔助選擇,使得到驗證,表明此標記可用於大豆耐鹽種質鑒定及大豆耐鹽遺傳育種的分子標記輔助選擇,使大豆耐鹽性室內鑒定成爲可能。爲此,大豆耐鹽性基因的分子標記及其獲得方法和應用已申請了中華人民共和國發明專利。

English Abstract

The purpose of this study was to screen and identify PCR markers associated with salt tolerant gene in soybean so that salt identification can be conducted efficiently and accurately. Soybean cultivars with salt tolerance or susceptibility and three crosses of salt tolerant cultivar with susceptible cultivar were used as materials in this experiment. By using BSA method, two codominant PCR markers were identified through the tolerant (susceptible) salt cultivars bulks and the tolerant (susceptible) salt bulks of a F2 population. There was a 600bp band in the susceptible individuals and a 700bp band or two 700bp/600bp bands in the tolerant individuals. The markers were closely linked with salt tolerant/susceptible alleles. Moreover the markers were tested in the other two ”salt tolerant cultivar×susceptible cultivar” F2 populations and confirmed by 12 salt tolerance cultivars and 13 salt susceptible cultivars with different genetic backgrounds. It indicated that the markers (700bp and 600bp) can be applied in salt tolerant identification of the soybean germplasm resources, and markers-assisted selection in salt tolerant breeding of soybean. The molecular markers, the obtained method and application of soybean salt tolerant gene were patented for invention in 1998.

Topic Category 生物農學 > 農業
生物農學 > 森林
生物農學 > 畜牧
生物農學 > 漁業