細胞核內蛋白質動態變化研究進展

Progress in Dynamic Studies of Nuclear Proteins

王愛俠(Ai-Xia Wang)；陳傑(Jie Chen)；劉紅林(Hong-Lin Liu)

蛋白質動態變化 ； 漂白後熒光恢復／漂白熒光丟失 ； 細胞核區域 ； 共價修飾 ； 基因調控 ； protein dynamics ； FRAP/FLIP ； nuclear compartment ； covalent modification ； gene regulation

生物化學與生物物理進展

31卷4期（2004 / 04 / 01）

304 - 308

簡體中文

漂白後熒光恢復和漂白熒光丟失技術是蛋白質動態變化研究中常用的兩項技術。近年來，利用這兩項技術對細胞核內蛋白質動態變化的研究表明：一些蛋白質在細胞核內是運動的，能和各自所在的區域快速結合和解離；並且這種運動主要以被動擴散的方式進行，不消耗代謝的能量；另外蛋白質的共價修飾可對某些蛋白質的運動產生影響。細胞核內蛋白質的動態變化對細胞核的結構組成和基因表達的調控都具有重要的意義，但詳細的機制還有待於進一步的研究。

FRAP and FLIP are two techniques that are usually applied to study the dynamics of protein in living cells. In recent years, studies of protein dynamics by these two techniques showed: some proteins are mobile, and they rapidly associate and dissociate with their respective compartments in the nucleus of living cells. Furthermore, protein movement is mainly independent of energy, which indicates that proteins may use a passive mechanism of movement. Additionally, covalent modifications have effect on the mobility of some proteins. Protein dynamics in the karyon is important to the nuclear architecture and gene regulation, however, the detail mechanism is still unknown and expected to further study.