Title

異煙肼耐藥結核分枝桿菌感染人源巨噬細胞的蛋白質組學研究

Translated Titles

Proteome Study of Macrophage Infected with Mycobacterium tuberculosis Isoniazid Resistant Strain

Authors

劉麗蓉(Li-Rong Liu);樂軍(Jun Yue);王洪海(Hong-Hai Wang)

Key Words

巨噬細胞U937 ; 耐藥 ; 結核分枝桿菌 ; 異煙肼 ; 蛋白質組 ; 差異表達 ; macrophage U937 ; resistance ; Mycobacterium tuberculosis ; isoniazid ; proteome ; differentially express

PublicationName

生物化學與生物物理進展

Volume or Term/Year and Month of Publication

31卷4期(2004 / 04 / 01)

Page #

361 - 367

Content Language

簡體中文

Chinese Abstract

利用雙向電泳技術,對人源巨噬細胞U937感染異煙肼耐藥結核分枝桿菌前後的全細胞蛋白表達圖譜進行差異比較和分析,發現其中產生差異的有32個蛋白質斑點,利用基質輔助激光解吸/電離飛行時間質譜技術,對其中5個表達明顯上調的蛋白質斑點進行分析鑒定,獲得5個明確的肽質量指紋圖譜,通過在數據庫中進行檢索分析,確定這5個蛋白質分別為熱休克蛋白105(下標 β)、凋亡抑制蛋白-1、磷酸甘油酸變位酶1、組織蛋白酶B、橋粒膠蛋白3。上述發現有助於瞭解耐藥結核分枝桿菌入侵早期導致的巨噬細胞蛋白質組表達變化,為深入研究耐藥結核分枝桿菌-宿主相互作用提供了探索方向。

English Abstract

Two-dimensional electrophoresis (2-DE) was employed to compare the global protein patterns between human macrophage cell line U937 infected and that uninfected with Mycobacterium tuberculosis (MTB) isoniazid (INH) resistant strains, and 32 protein spots were found to express differentially. Five protein spots which remarkably increased in U937 infected with MTB INH-resistant strains were measured by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The data obtained from peptide mass fingerprinting were used in protein database search. Five protein spots in gel were identified as HSP105(subscript β), inhibitor of apoptosis protein-1, phosphoglycerate mutase 1, cathepsin B, desmocollin 3. These data provide insight into the changed global protein patterns of the U937 while in stasis shortly after infection and may prove useful for further study in the interaction between MTB INH-resistant strains and host.

Topic Category 基礎與應用科學 > 物理
基礎與應用科學 > 化學
生物農學 > 生物科學