Title

黃芪中黃酮類組分的高效液相色譜分離條件的快速優化

Translated Titles

Quick Optimization of High Performance Liquid Chromatographic Conditions for Separation of Flavones of Astragalus

Authors

徐青(Qing Xu);王龍星(Long-Xing Wang);張曦(Xi Zhang);金高娃(Gao-Wa Jin);肖紅斌(Hong-Bin Xiao);梁鑫淼(Xin-Miao Liang)

Key Words

高效液相色譜法 ; 色譜條件的優化 ; 重疊分辨圖 ; 黃酮類組分 ; 黃芪 ; high performance liquid chromatography ; chromatographic condition optimization ; overlapping separation range map ; flavones ; Astragalus

PublicationName

色譜

Volume or Term/Year and Month of Publication

23卷6期(2005 / 11 / 01)

Page #

630 - 632

Content Language

簡體中文

Chinese Abstract

以黃芪為研究對象,對黃芪中乙酸乙酯部位的化學成分進行了高效液相色譜分析並對其色譜操作條件進行了快速優化。根據幾個線性梯度下的保留時間來計算各組分的保留參數,然後利用重疊分辨圖法確定其最佳分析條件。在選定的最佳條件下各組分分離情況良好。利用梯度保留時間計算保留參數比較方便快速,並可以有效地避免以往等度線性回歸法遇到的峰識別問題。該方法更適用於實際複雜樣品色譜分析條件的優化。

English Abstract

An analytical method for flavones of Astragalus has been developed and a process of high performance liquid chromatographic (HPLC) condition optimization has been elaborated. The LC retention parameters of 34 components were obtained quickly by using three linear gradient elutions. Overlapping separation range map (OSRM), which is related to the separation quality and the analysis time, was utilized to search the optimised stepwise gradient method for HPLC analysis of flavones of Astragalus. Finally, the optimised stepwise gradient elution conditions were set up with the help of this map. Under the optimised conditions, the sample of flavones of Astragalus was well separated in reasonable time. The method is more convenient than that using a series of isocratic elutions.

Topic Category 基礎與應用科學 > 化學