Translated Titles

Quick Optimization of High Performance Liquid Chromatographic Conditions for Separation of Flavones of Astragalus


徐青(Qing Xu);王龍星(Long-Xing Wang);張曦(Xi Zhang);金高娃(Gao-Wa Jin);肖紅斌(Hong-Bin Xiao);梁鑫淼(Xin-Miao Liang)

Key Words

高效液相色譜法 ; 色譜條件的優化 ; 重疊分辨圖 ; 黃酮類組分 ; 黃芪 ; high performance liquid chromatography ; chromatographic condition optimization ; overlapping separation range map ; flavones ; Astragalus



Volume or Term/Year and Month of Publication

23卷6期(2005 / 11 / 01)

Page #

630 - 632

Content Language


Chinese Abstract


English Abstract

An analytical method for flavones of Astragalus has been developed and a process of high performance liquid chromatographic (HPLC) condition optimization has been elaborated. The LC retention parameters of 34 components were obtained quickly by using three linear gradient elutions. Overlapping separation range map (OSRM), which is related to the separation quality and the analysis time, was utilized to search the optimised stepwise gradient method for HPLC analysis of flavones of Astragalus. Finally, the optimised stepwise gradient elution conditions were set up with the help of this map. Under the optimised conditions, the sample of flavones of Astragalus was well separated in reasonable time. The method is more convenient than that using a series of isocratic elutions.

Topic Category 基礎與應用科學 > 化學