Title

毛細管電泳法研究環糊精與藥物蘆丁和氯黴素的相互作用

Translated Titles

Investigation on Interactions of Rutin and Chloramphenicol with β-Cyclodextrin Using Capillary Electrophoresis

Authors

傅崇崗(Chong-Gang Fu);楊冬芝(Dong-Zhi Yang)

Key Words

毛細管電泳 ; β-環糊精 ; 蘆丁 ; 氯黴素 ; 包合常數 ; 熱力學參數 ; capillary electrophoresis ; β-cyclodextrin ; rutin ; chloramphenicol ; inclusion constants ; thermodynamic parameters

PublicationName

色譜

Volume or Term/Year and Month of Publication

23卷6期(2005 / 11 / 01)

Page #

673 - 676

Content Language

簡體中文

Chinese Abstract

採用毛細管電泳淌度移動法測定了β-環糊精與蘆丁和氯黴素這兩種藥物的包合常數及包合反應的熱力學參數。所用未塗層熔融石英毛細管的規格為內徑50μm,總長37cm,有效長度30cm,電泳緩衝液為25mmol/L硼砂+100mmol/L硼酸(pH8.6),並加入不同濃度的β-CD。在25℃時測得蘆丁和氯黴素與β-CD的包合常數分別為98.5和1204L/mol。通過測定不同溫度下的包合常數,求得包合反應的系列熱力學參數。結果表明β-CD與兩種藥物的包合反應焓熵互補現象明顯。採用MM2力場對β-CD與兩種藥物分子的包合物的穩定構型進行了分子力學模擬,模擬結果與實驗結果相符。

English Abstract

The interactions of two medicines of rutin and chloramphenicol with β-cyclodextrin were investigated using mobility shift method by capillary electrophoresis. The size of the capillary column was 37cm×50Rm i. d. with an effective length of 30cm. The running buffer was a mixture of 25mmol/L sodium borate and 100mmol/L boric acid (pH 8.6). Effective mobilities of the two medicines changed with altering the concentration of β-cyclodextrin. By nonlinear least square fitting curve, the inclusion constants of 98.5 and 1204L/mol for rutin and chloramphenicol were obtained at 25℃ respectively. The inclusion constants at different temperatures were determined, from which a series of thermodynamic parameters were derived. Experimental results show that an obvious relationship of mutual compensation between enthalpy and entropy existed in these inclusion reactions. The most stable configurations of the inclusions were calculated using MM2 force field, and the results were well in agreement with the experimental results.

Topic Category 基礎與應用科學 > 化學