Translated Titles

Investigation on Interactions of Rutin and Chloramphenicol with β-Cyclodextrin Using Capillary Electrophoresis


傅崇崗(Chong-Gang Fu);楊冬芝(Dong-Zhi Yang)

Key Words

毛細管電泳 ; β-環糊精 ; 蘆丁 ; 氯黴素 ; 包合常數 ; 熱力學參數 ; capillary electrophoresis ; β-cyclodextrin ; rutin ; chloramphenicol ; inclusion constants ; thermodynamic parameters



Volume or Term/Year and Month of Publication

23卷6期(2005 / 11 / 01)

Page #

673 - 676

Content Language


Chinese Abstract


English Abstract

The interactions of two medicines of rutin and chloramphenicol with β-cyclodextrin were investigated using mobility shift method by capillary electrophoresis. The size of the capillary column was 37cm×50Rm i. d. with an effective length of 30cm. The running buffer was a mixture of 25mmol/L sodium borate and 100mmol/L boric acid (pH 8.6). Effective mobilities of the two medicines changed with altering the concentration of β-cyclodextrin. By nonlinear least square fitting curve, the inclusion constants of 98.5 and 1204L/mol for rutin and chloramphenicol were obtained at 25℃ respectively. The inclusion constants at different temperatures were determined, from which a series of thermodynamic parameters were derived. Experimental results show that an obvious relationship of mutual compensation between enthalpy and entropy existed in these inclusion reactions. The most stable configurations of the inclusions were calculated using MM2 force field, and the results were well in agreement with the experimental results.

Topic Category 基礎與應用科學 > 化學