Title

聚乙二醇对血红蛋白离子交换层析行为的影响

Translated Titles

Effects of Polyethylene Glycol on the Ion Exchange Chromatographic Behaviors of Hemoglobin

Authors

赵东旭(Dong-Xu Zhao);路秀玲(Xiu-Ling Lu);苏志国(Zhi-Guo Su)

Key Words

血红蛋白 ; 聚乙二醇 ; 离子交换层析 ; 共溶剂 ; hemoglobin ; polyethylene glycol ; ion exchange chromatography ; cosolvent

PublicationName

北京理工大學學報

Volume or Term/Year and Month of Publication

24卷10期(2004 / 10 / 01)

Page #

924 - 928

Content Language

簡體中文

Chinese Abstract

研究作为共溶剂的聚乙二醇(PEG600和PEG4000)在离子交换层析法纯化血红蛋白过程中对血红蛋白层析行为的影响。预处理后的牛血红细胞破裂液用Q Sepharose Big Beads阴离子交换介质进行层析。层析过程中添加质量分数为0.5%~5.0%的PEG600或PEG4000,血红蛋白回收率由76%提高到90%以上,血红蛋白纯度略有增加并且其生物活性得到较好地保持,PEG600对血红蛋白层析的保留时间影响不大,PEG4000使血红蛋白的层析保留时间增加,增加强度与PEG的浓度之间呈正相关,并用共溶剂理论对上述结果进行了讨论。

English Abstract

Effects of polyethylene glycol (PEG600 and PEG4000) on the ion exchange chromatographic behaviors of bovine hemoglobin (Hb) was studied. Pretreated bovine hemolysate was chromatographied with Q Sepharose Big Beads, and 0.5%~5.0% PEG600 or PEG4000 was added as a cosolvent. Consequently, the purity of Hb increased slightly, near to electrophoretical purity. The recovery of Hb was obviously higher than the control (76%), especially in the presence of PEG4000, which was over 90%. Bioactivity of Hb was well preserved in the process of chromatography after adding the cosolvent. The retention time of Rb in chromatography was not affected when PEG600 was added as the cosolvent, while it was longer in the presence of PEG4000 and the time increased as the concentration of PEG4000 improved. The results were discussed based on the theory of cosolvent.

Topic Category 基礎與應用科學 > 基礎與應用科學綜合
工程學 > 工程學綜合