Title

應用Steedman's wax切片法觀察植物細胞微管骨架

Translated Titles

Steedman's Wax Sectioning for Observation of the Microtubule Cytoskeleton in Plant Cells

Authors

何群(Qun He);尤瑞麟(Rui-Lin You)

Key Words

Steedman's wax ; 植物細胞 ; 微管骨架 ; Steedman's wax ; Plant cells ; Microtubule cytoskeleton

PublicationName

植物學通報

Volume or Term/Year and Month of Publication

21卷5期(2004 / 10 / 01)

Page #

547 - 555

Content Language

簡體中文

Chinese Abstract

微管骨架在植物發育過程中起重要作用。由於植物細胞的特殊性,與動物細胞相比植物微管骨架的研究遇到更多的困難。簡略地介紹了曾被國內外學者應用的植物微管骨架的各種研究方法及其局限性。Steedman's wax是一種多脂蠟。它熔點低(35~37℃),具有與石蠟相同的切片性質,能夠切成不同厚度的連續切片,適合深埋於器官內部的組織或細胞的免疫細胞化學研究。介紹了應用Steedmam's wax切片法觀察植物細胞微管骨架的一般程序和方法以及經過作者檢驗且切實可行的一些技術改進。

English Abstract

As one of the major components of the cytoskeleton, the microtubule cytoskeleton plays an important role in plant development The present paper gives a brief introduction on the techniques widely applied in this field and their limitations, and recommends a method, a low melting polyester wax (steedman's wax) as an embedding medium for the investigation of the microtubule cytoskeleton with immuno-cytochemistry Its low melt point (35-37℃) makes it favorable for keeping the antigenicity Chlorophyll is extracted in the process of infiltration so that the interference of intense autofluorescence from chloroplasts decreases Steedman's wax has similar characters with paraffin, being able to cut into ribbon-sections of different thickness, which is beneficial both to antibody penetration because of the breaking of cell wall and to the observation of successive focal planes (in ribbon-sections) with confocal laser scanning microscopy General procedures of Steedman's w ax sectioning and some practical improvements were also discussed in the present paper

Topic Category 生物農學 > 植物學
生物農學 > 農業