海蒿子多糖的提取方法研究

The Research on the Extraction Methods of Crude Polysaccharide from Sargassum Pallidium

赵宇(Yu Zhao)；李志富(Zhi-Fu Li)；任少红(Shao-Hong Ren)；陈小全(Xiao-Quan Chen)

海蒿子 ； 粗多糖 ； 水提取 ； 酸提取 ； sargassum pallidium ； crude polysaccharide ； water-extraction ； acid-extraction

泰山醫學院學報

25卷5期（2004 / 10 / 25）

429 - 430

簡體中文

目的　比较两种海蒿子粗多糖的提取方法。方法　海蒿子干粉经热水抽提，乙醇沉淀，得到水溶性的粗多糖C1。用稀盐酸室温提取，乙醇沉淀，得到粗多糖C2。对两种粗品用苯酚硫酸法测其总糖含量，BaCl2明胶法测其硫酸根含量。紫外光谱检测C1、C2核酸和蛋白吸收情况。结论　从总糖含量及蛋白质吸收峰看，水提方法好于酸提方法；从硫酸根含量及粗多糖产率看酸提方法好于水提方法。

Objective: To compare the two methods of crude polysaccharide from Sargassum Pallidium. Methods: The crude polysaccharides (C1、C2) was obtained from the Sargassum Pallidium power by boilngwater-extracting, acid-extracting under room temperature, and ethanol-precipitating. Their total polysaccharides content was determined by the method of phenol-sulphuric acid. Their total sulfate content was determined by the method of BaCl2 -glutin. The absorbance of nucleic acid and protein of these two samples was detected by ultraviolet spectrum. Results: To the total content of the polysaccharides and protein absorbance, the water-extracting method is better than the acid-extracting; to the content of SO4(superscript 2-) and productivity, the acid-extracting method is better than the water-extracting.