Title

海蒿子多糖的提取方法研究

Translated Titles

The Research on the Extraction Methods of Crude Polysaccharide from Sargassum Pallidium

Authors

赵宇(Yu Zhao);李志富(Zhi-Fu Li);任少红(Shao-Hong Ren);陈小全(Xiao-Quan Chen)

Key Words

海蒿子 ; 粗多糖 ; 水提取 ; 酸提取 ; sargassum pallidium ; crude polysaccharide ; water-extraction ; acid-extraction

PublicationName

泰山醫學院學報

Volume or Term/Year and Month of Publication

25卷5期(2004 / 10 / 25)

Page #

429 - 430

Content Language

簡體中文

Chinese Abstract

目的 比较两种海蒿子粗多糖的提取方法。方法 海蒿子干粉经热水抽提,乙醇沉淀,得到水溶性的粗多糖C1。用稀盐酸室温提取,乙醇沉淀,得到粗多糖C2。对两种粗品用苯酚硫酸法测其总糖含量,BaCl2明胶法测其硫酸根含量。紫外光谱检测C1、C2核酸和蛋白吸收情况。结论 从总糖含量及蛋白质吸收峰看,水提方法好于酸提方法;从硫酸根含量及粗多糖产率看酸提方法好于水提方法。

English Abstract

Objective: To compare the two methods of crude polysaccharide from Sargassum Pallidium. Methods: The crude polysaccharides (C1、C2) was obtained from the Sargassum Pallidium power by boilngwater-extracting, acid-extracting under room temperature, and ethanol-precipitating. Their total polysaccharides content was determined by the method of phenol-sulphuric acid. Their total sulfate content was determined by the method of BaCl2 -glutin. The absorbance of nucleic acid and protein of these two samples was detected by ultraviolet spectrum. Results: To the total content of the polysaccharides and protein absorbance, the water-extracting method is better than the acid-extracting; to the content of SO4(superscript 2-) and productivity, the acid-extracting method is better than the water-extracting.

Topic Category 醫藥衛生 > 醫藥衛生綜合
醫藥衛生 > 預防保健與衛生學
醫藥衛生 > 藥理醫學
醫藥衛生 > 社會醫學