Title

發育中玉米胚結合蛋白(BiP)的動態變化及定位

Translated Titles

Changes and Localization of Binding Protein (BiP) during Maize Embryo Development

Authors

林曉東(Xiao-Dong Lin);傅家瑞(Jia-Rui Fu);黃上志(Shang-Zhi Huang)

Key Words

玉米 ; 熱激蛋白 ; 種子發育 ; 結合蛋白 ; Maize Zea mays L. ; Heat shock protein ; Seed development ; Binding protein

PublicationName

熱帶亞熱帶植物學報

Volume or Term/Year and Month of Publication

12卷2期(2004 / 03 / 01)

Page #

159 - 162

Content Language

簡體中文

Chinese Abstract

Western blot檢測表明,在玉米胚發育過程中結合蛋白(BiP)含量與胚可溶性蛋白含量變化一致,在授粉16d後BiP含量隨發育而增加:對熱激不敏感。組織化學免疫定位表明,在玉米胚發育的不同時期,BiP主要定位在胚芽端、初生維管組織和糊粉層中,提示胚在構建器官的同時,也為其功能執行準備了條件;熱激不影響其定位。

English Abstract

Western blot showed that binding protein(BiP) kept a high expression level during maize embryo development, which coincided with the change in embryo soluble protein. The content of soluble protein rapidly increased with the embryo development after pollination for 16 days, while BiP markedly increased from 22nd day after pollination. The BiP failed to respond to heat shock. Immunohistochemical localization showed that BiP localized in plumule, primary vascular tissue and aleurone layer during embryo development.

Topic Category 生物農學 > 植物學
生物農學 > 生物環境與多樣性