Title

大肠杆菌致U937细胞凋亡的研究

Translated Titles

Effect of E.coli on Cell Apoptosis of U937

Authors

王佳贺(Jia-He Wang);陈佰义(Bai-Yi Chen);周一军(Yi-Jun Zhou);邝栋明(Dong-Ming Kuang);郑利民(Li-Min Zheng)

Key Words

大肠杆菌 ; 细胞凋亡 ; U937 ; E.coli ; apoptosis ; U937

PublicationName

中國現代醫學雜誌

Volume or Term/Year and Month of Publication

15卷22期(2005 / 11 / 30)

Page #

3375 - 3377

Content Language

簡體中文

Chinese Abstract

[Objective] To study the time-dependent manner of U937 apoptosis induced by E.coli. [Methods] U937 apoptosis was detected with Annexin V/PI assay and morphological detectionat different time after E.coli infecting U937. [Results] Morphologic observation showed that U937 cells in the 60 min and 90min groups underwent karyopykosis, condensation, swelling and apoptotic bodies were observed. In the 20 min and 30 min groups, very few apoptotic cells were observed. Annexin V/PI assay showed that the percentage of apoptotic cells was raised with the increasing of time. the infection group at 60min and 90min can induce significantly U937 apoptosis than in the other groups (P<0.001). [Conclusion] E.coli induces U937 apoptosis in time-dependent manner.

English Abstract

[Objective] To study the time-dependent manner of U937 apoptosis induced by E.coli. [Methods] U937 apoptosis was detected with Annexin V/PI assay and morphological detectionat different time after E.coli infecting U937. [Results] Morphologic observation showed that U937 cells in the 60 min and 90min groups underwent karyopykosis, condensation, swelling and apoptotic bodies were observed. In the 20 min and 30 min groups, very few apoptotic cells were observed. Annexin V/PI assay showed that the percentage of apoptotic cells was raised with the increasing of time. the infection group at 60min and 90min can induce significantly U937 apoptosis than in the other groups (P<0.001). [Conclusion] E.coli induces U937 apoptosis in time-dependent manner.

Topic Category 醫藥衛生 > 醫藥衛生綜合
醫藥衛生 > 預防保健與衛生學
醫藥衛生 > 藥理醫學
醫藥衛生 > 社會醫學