Inhibitory Effects of the Part C in Alcohol Extract of Verbena Officinalis on Human Choriocarcinoma JAR Cell Line
張立平(Li-Ping Zhang)；羅莉(Li Luo)；王家俊(Jia-Jun Wang)；徐昌芬(Chang-Fen Xu)
絨毛膜癌 ； JAR ； 馬鞭草提取液 ； 細胞增殖 ； choriocarcinoma ； JAR ； Alcohol Extract of Verbena officinalis ； Proliferation
|Volume or Term/Year and Month of Publication||
24卷5期（2004 / 09 / 05）
470 - 472
Objective: To investigate the effect of the Part C in Alcohol Extract of Verbena officinalis on Human Choriocarcinoma JAR Cell Line. Methods: Firstly, morphological changes of JAR cells were observed with phase-contrst microscope. Secondly, JAR cells were determined in the proliferation-inhibition ratio of the part C with vary chroma (luring different period of time using MTT method. And thirdly, flow cytometry (FCM) was performed for measurement of phase of cell cycle. Results: The incubated tumor cells were decreased in number and became atrophic when they were treated with the part C. The inhibition rate of experimental groups was more than that in control group and increased according to increasement of time and concentration. In 72 h 40 mg/ml, there was a significant control and its inhibition was 65%. Phase of cell cycle analysis by FCM showed that cells were arrested in G2/M phase. Conclusion: The part C in Alcohol Extract of Verbena officinalis is a highly effective antineoplastic agent to choriocarcinoma cells, manifestating time-concentration effect.
醫藥衛生 > 藥理醫學