Title

JWA蛋白與α-微管蛋白結合和對微管穩定性的調節

Translated Titles

JWA Protein Binds to α-tubulin and Regulates Microtubule Stability

Authors

陳海蓉(Hai-Rong Chen);李愛群(Ai-Qun Li);李愛萍(Ai-Ping Li);周建偉(Jian-Wei Zhou)

Key Words

JWA蛋白 ; α-微管蛋白 ; 微管相關蛋白 ; 有絲分裂 ; JWA protein ; α-tubulin ; cytoskeleton

PublicationName

南京醫科大學學報(自然科學版)

Volume or Term/Year and Month of Publication

24卷5期(2004 / 09 / 05)

Page #

519 - 521

Content Language

簡體中文

Chinese Abstract

目的:探討了JWA蛋白與α-微管蛋白在細胞內的分佈和相互關係,特別是在有絲分裂過程中的動態變化及與微管蛋白的關係。方法:用熒光顯微鏡技術研究低溫處理、處理後恢復的NIH3T3細胞JWA蛋白和微管蛋白的相互作用;用激光共聚焦技術檢測MH3T3細胞的JWA蛋白與α-微管蛋白在細胞內的分佈。結果:JWA蛋白在細胞內與α-微管蛋白的分佈基本平行。結論:JWA蛋白,作為一種新的微管相關蛋白,在微管動力學變化過程和有絲分裂過程中與α-微管蛋白有交互作用,與α-微管蛋白結合,可能對微管穩定性起一定的調節作用。

English Abstract

Objective: JWA gene is a cytoskeleton related gene and associated with cell differentiation and apoptosis. Our recent studies have demonstrated that JWA protein is not only structurally binding to β-tubulin, hut a novel functional MAP-contributions to cell shape and cellular amino acid transportation. Methods: To understand if JWA protein is localized to α-tubulin, in this study, we use immunofluorescence microscopy assay for NIH3T3 cell culture models and the results have provided strung evidence for a linkage between JWA protein and α-tubulin. Results: Briefly, JWA protein is stably binding to α-tubulin whenever α-tubulin is temperature-dependently polymerized or not, and it might be critical to the mitosis of the cell. The confocal laser scanning microscopy revealed that the intracellular distribution of JWA protein is obviously different to that of α-tubulin during mitosis. Conclusion: These results suggest that JWA protein binds to α-tubulin, and might be a novel modulator of microtubule.

Topic Category 醫藥衛生 > 醫藥衛生綜合
醫藥衛生 > 藥理醫學