Title

鋅對人前列腺癌細胞增殖的影響及機制初步探討

Translated Titles

Effect of Zinc on Human Prostatic Carcinoma Cell Growth

Authors

徐志兵(Zhi-Bing Xu);王國民(Guo-Min Wang);張永康(Yong-Kang Zhang);孫立安;金泰廙

Key Words

鋅 ; 前列腺癌 ; 增殖 ; Zinc ; Prostatic carcinoma ; Growth

PublicationName

中國臨床醫學

Volume or Term/Year and Month of Publication

12卷4期(2005 / 08 / 25)

Page #

636 - 638

Content Language

簡體中文

Chinese Abstract

目的:瞭解鋅對人前列腺癌細胞增殖的影響。方法:採用不同濃度鋅溶液干預人前列腺癌細胞株體外培養,測定細胞生長曲線,檢測細胞生長抑制情況、細胞存活率、細胞凋亡及細胞週期情況。結果:鋅對雄激素非依賴型(DU-145和PC-3)及雄激素依賴型(LNCaP)前列腺癌細胞均有抑制增殖和促進凋亡作用,對激素依賴型抑制更明顯,3種細胞的IC50分別為780ng/ml、810ng/ml、100ng/ml。1000ng/ml鋅對3種細胞生長均達最大抑制作用。隨著鋅濃度增高,3種細胞凋亡率均上升。結論:鋅可以明顯抑制雄激素非依賴型以及雄激素依賴型前列腺癌細胞的增殖,可能與鋅誘導細胞凋亡有關。

English Abstract

Objective: To evaluate the effect of zinc on human proatatic carcinoma (PCa) cell growth. Methods: Growth inhibition and various regulatory responses were investigated in three human prostatic carcinoma cell lines (DU-145, PC-3 and LNCaP), treated with various concentrations of zinc. Results: Incubation of the PCa cell lines with different levels of zinc resulted in inhibition of cell growth, a lower 50% inhibition of cell growth (IC50) value for zinc(about 100 ng/ml)was detected in LNCaP cells, which were androgen dependent, whereas androgen independent PC-3 and DU-145 cells exhibited a higher IC50 for zinc (about 780 ng/ml and 810 ng/ml). Incubation with 1 000 ng/ ml zinc resulted in maximum inhibition of growth in both cell lines. Cell flow cytometric analyses revealed that the cell growth inhibitions were accompanied by an increase in apoptosis. Conclusion: These results suggest that zinc inhibits human prostatic carcinoma cell growth, possibly due to induction of cell apoptosis.

Topic Category 醫藥衛生 > 醫藥衛生綜合