Title

骨髓增生異常綜合徵患者Th1/Th2細胞平衡改變的臨床意義

Translated Titles

The Clinical Significance of Balance between Type Ⅰ and Type Ⅱ Immune Responses of Lymphocyte Subpopulations in Myelodysplastic Syndrome

Authors

楊雋(Jun Yang);王椿(Chun Wang);謝匡成(Kuang-Cheng Xie);顏式可;高彥榮;蔡琦;秦尤文;萬理萍;蔡宇

Key Words

骨髓增生異常綜合徵 ; Th1 ; Th2 ; 流式細胞術 ; Myelodysplastic syndrome ; Th1 ; Th2 ; Flow cytometry

PublicationName

中國臨床醫學

Volume or Term/Year and Month of Publication

12卷4期(2005 / 08 / 25)

Page #

705 - 708

Content Language

簡體中文

Chinese Abstract

Objective: To evaluate the clinical significance of the subsets of CD4(superscript +), CD8(superscript +) cells and the relationship of the activated T cells in peripheral blood from patients with myelodysplastic syndrome (MDS). Methods: We investigated the intracytoplasmic expression on CD4(superscript +) and CD8(superscript +) T cells after in vitro cultivation with PMA, Ionomycin and Monensin in 14 patients with RA and 1 patient with RAEB. Flow cytometry was used for the Counts of CD3(superscript +) CD8(superscript +) IFN-((superscript +) cells (Th1), CD3(superscript +) CD8(superscript +) IL-4(superscript +) cells (Th2), CD8(superscript +) IFN-((superscript +) cells (Tc1), CD3(superscript +) CD8(superscript +) IL-4(superscript +) cells (Tc2). Furthermore, we studied the relationship between the Th cells, Tc cells and the activated T cells. Results: The percentages of Th1 cells in peripheral blood from patients with MDS were higher than those of control group. Th cells were deviated to the Th1 cells in the body of MDS The increased production of cytokines in MDS which inhibit hematopoiesis such as IFN-γ, TNF-β might reflect deviations of Th distributions. There were no significant differences in the proportion of Th2, Tc1 and Tc2 cells. The relationship between the Th1 cells and CD4(superscript +) CD45RO(superscript +) Cells was positive (r=0.573, P<0.05), while Th1 Cells versus CD4(superscript +) CD45RO(superscript +) cells was negative (r=-0.509, P<0.05). There was no relationship between the expression rates of Th2, Tc1, Tc2 cells and CD4(superscript +) CD45RO(superscript +) cells(P>0.05). Conclusion: There was the imbalanced development of Th cells and the overproduction of cytokines inhibitory to hematopoiesis in MDS. T lymphocytes played an important role in producing these cytokines.

English Abstract

Objective: To evaluate the clinical significance of the subsets of CD4(superscript +), CD8(superscript +) cells and the relationship of the activated T cells in peripheral blood from patients with myelodysplastic syndrome (MDS). Methods: We investigated the intracytoplasmic expression on CD4(superscript +) and CD8(superscript +) T cells after in vitro cultivation with PMA, Ionomycin and Monensin in 14 patients with RA and 1 patient with RAEB. Flow cytometry was used for the Counts of CD3(superscript +) CD8(superscript +) IFN-((superscript +) cells (Th1), CD3(superscript +) CD8(superscript +) IL-4(superscript +) cells (Th2), CD8(superscript +) IFN-((superscript +) cells (Tc1), CD3(superscript +) CD8(superscript +) IL-4(superscript +) cells (Tc2). Furthermore, we studied the relationship between the Th cells, Tc cells and the activated T cells. Results: The percentages of Th1 cells in peripheral blood from patients with MDS were higher than those of control group. Th cells were deviated to the Th1 cells in the body of MDS The increased production of cytokines in MDS which inhibit hematopoiesis such as IFN-γ, TNF-β might reflect deviations of Th distributions. There were no significant differences in the proportion of Th2, Tc1 and Tc2 cells. The relationship between the Th1 cells and CD4(superscript +) CD45RO(superscript +) Cells was positive (r=0.573, P<0.05), while Th1 Cells versus CD4(superscript +) CD45RO(superscript +) cells was negative (r=-0.509, P<0.05). There was no relationship between the expression rates of Th2, Tc1, Tc2 cells and CD4(superscript +) CD45RO(superscript +) cells(P>0.05). Conclusion: There was the imbalanced development of Th cells and the overproduction of cytokines inhibitory to hematopoiesis in MDS. T lymphocytes played an important role in producing these cytokines.

Topic Category 醫藥衛生 > 醫藥衛生綜合