Expressions of KiSS-1 Gene and Matrix metalloproteinase-9 in Gestational Trophoblast Disease and Their Significance
乔宠(Chong Qiao)；王春晖(Chun-Hui Wang)；刘贵鹏(Gui-Peng Liu)；程大丽(Da-Li Cheng)；张淑兰(Shu-Lan Zhang)
妊娠并发症，肿瘤／病理学 ； 滋养层肿瘤／病理学 ； 基因表达 ； 金属蛋白酶类／生物合成 ； pregnancy complications, neoplastic/pathology ； trophoblastic neoplasms/pathology ； gene expression ； metalloproteinases/biosynthesis
|Volume or Term/Year and Month of Publication||
12卷13期（2005 / 07 / 14）
994 - 998
目的：探讨浸润相关基因KiSS-1和基质金属蛋白酶－9(matrix metalloproteinase, MMP-9)在妊娠滋养细胞疾病中的表达。方法：用RT-PCR和Western blot法检测30例正常早期妊娠绒毛、30例葡萄胎、9例侵蚀性葡萄胎和8例绒毛膜癌中KiSS-1和MMP-9mRNA及其蛋白的表达。结果：正常妊娠早期绒毛、葡萄胎和侵蚀性葡萄胎组织中均有MMP-9和KiSS-1表达，而在绒毛膜癌组织中仅有MMP-9基因和蛋白的表达，无KiSS-1基因和其蛋白肽metastin的表达。妊娠滋养细胞疾病组织［包括葡萄胎、侵蚀性葡萄胎及绒毛膜癌］中MMP-9的基因和蛋白表达均显著高于早期妊娠绒毛组织(P=0.039、0.001、0.000)，其中侵蚀性葡萄胎及绒毛膜癌组织中显著高于葡萄胎(P=0.000)，绒毛膜癌中MMP-9基因表达水平最高（分别为0.705±0.141和78.403±7.124）。而KiSS-1基因的表达正相反：葡萄胎组织的KiSS-1 mRNA(0.433±0.193)和metastin表达(23.831±7.522)显著低于早期妊娠绒毛组织(P=0.049、0.049)，侵蚀性葡萄胎组织中KiSS-1 mRNA和metastin表达水平更低（分别为0.113±0.121和10.814±3.431）。结论：促浸润基因MMP-9的表达变化与滋养细胞浸润活性呈正相关，而抑浸润基因KiSS-1的表达变化则与滋养细胞浸润活性呈负相关。这两种基因相互作用在滋养细胞浸润活性调控中起重要作用。
OBJECTIVE: To investigate the expressions of metastasis related gene-KiSS-1 and matrix metalloproteinase (MMP-9) in gestational trophoblastic disease (GTD) and their significance. METHODS: RT-PCR analysis and Western blot analysis were used to detect KiSS-1 and MMP-9 expression levels in tissues of 30 cases of first-trimester villi, 30 cases of hydatidiform mole, 9 cases of invasive mole and 8 cases of choriocarcinoma. RESULTS: Expressions of MMP-9 and KiSS-1 were detected in tissues of first-trimester villi, hydatidiform mole and invasive mole. However, expression of MMP-9 was detected in tissues of chriocarcinoma, in which expressions of KiSS-1 gene and its protein peptide-metastin were not detected. The expressions of MMP-9 mRNA and protein in tissues of GTD, which included hydatidiform mole, invasive mole and choriocarcinoma, were significantly higher than those in villi of first-trimester (P=0.039, 0.001, 0.000). The expression level of MMP-9 in tissues of invasive mole and choriocarcinoma was higher than that of hyddatidiform mole significantly (P=0.000). The mRNA and protein levels of MMP-9 in tissues of choriocarcinoma were the highest (0.705±0.141 and 78.403±7.124, respectively), while the expression of KiSS-1 was contrary: The expression of KiSS-1mRNA (0.433±0.193) and metastin (23.831±7.522) in tissues of hyddatidiform mole were lower than those in villi of first-trimester (P=0.049, 0.049). The expression levels of KiSS-1 mRNA and metastin in tissues of invasive mole and choriocarcinoma were lower (0.113±0.121 and 10.814±3.431, respectively). CONCLUSIONS: The expression of invasion related gene, MMP-9, is positive relative to invasive ability of trophoblast, while the invasion suppressor gene, KiSS-1, is negative relative to invasive ability of trophoblast. The coeffection of these two genes plays an important role in regulating the invasion of trophoblast.